关键词: 关键词： 结直肠癌, LncRNA EVADR, 增殖, 迁移

Abstract: Objective To explore the effect and mechanism of lncRNA EVADR on the proliferation and migration in human colorectal cancer cell lines HCT116 and LOVO. Methods HCT116 and LOVO cell lines were transfected with LncRNA EVADR by overexpressing lentivirus system. CCK8 assay was performed to measure the growth of HCT116 and LOVO cells after overexpression of EVADR. Transwell migration was performed to determine if EVADR could promote HCT116 and LOVO cells migration. Finally, the expressions of E-cadherin and transcription factor Snail, Slug, ZEB1 and ZEB2 were detected by Western blot and real - time quantitative PCR respectively. Results We successfully established colorectal cancer cells strains HCT116, LOVO which can stably overexpress LncRNA EVADR and the capacity of proliferation and migration in overexpression group were significantly improved (P <0.05). The expression of E-cadherin was decreased while mesenchymal markers Snail, Slug, ZEB1 and ZEB2 were increased in EVADR overexpression HCT116 and LOVO cells. Conclusion Overexpression of LncRNA EVADR in HCT116 and LOVO cells can significantly promote the proliferation and migration of HCT116 and LOVO cells, which may play a important role in regulating epithelial-mesenchymal transition in colorectal cancer cells.

Key words: Key words: Colorectal cancer, LncRNA EVADR, Proliferation, migration