关键词: 紫杉醇, 奥沙利铂, 非小细胞肺癌, 细胞凋亡, 程序性细胞死亡蛋白-5（PDCD5）, X-连锁凋亡抑制蛋白（XIAP）

Abstract: Objective To investigate the therapeutic effect of paclitaxel plus oxaliplatin chemotherapy to the transplanted non-small cell lung cancer of nude mice and the effect to the apoptosis protein expression of PDCD5 and XIAP. Methods Building the nude mice model of transplanted lung cancer and all tumor-bearing mice were randomly divided into blank group, normal saline group, oxaliplatin group, paclitaxel group, paclitaxel plus oxaliplatin group. The gene expression of PDCD5 and XIAP were assayed by real-time quantitative PCR (q-PCR). The apoptosis related PDCD5 and XIAP protein expression was detected by Western blot. Finally, the tumor weight of each group was measured for statistical analysis. Results The mRNA expression of PDCD5 was highest and the gene expression of XIAP was lowest in paclitaxel plus oxaliplatin group (P < 0.01). The expression of PDCD5 protein was highest and the expression of XIAP protein was lowest in paclitaxel plus oxaliplatin group (P < 0.01). Finally, compare the tumor weight of each group, paclitaxel plus oxaliplatin group has the least mass (P < 0.01). Conclusion: Paclitaxel plus oxaliplatin group chemotherapy can significantly increase PDCD5 expression and reduce XIAP expression. Meanwhile, paclitaxel plus oxaliplatin chemotherapy can significantly reduce the tumor weight of happened non-small cell lung cancer.

Key words: Paclitaxel, Oxaliplatin, Non-small cell lung cancer, Cell apoptosis, Programmed Cell Death 5(PDCD5), X-linked inhibitor of apoptosis protein(XIAP)