跨膜蛋白66过表达减轻大鼠颈动脉球囊损伤后内膜增生

基础医学与临床 ›› 2017, Vol. 37 ›› Issue (6): 775-780.

跨膜蛋白66过表达减轻大鼠颈动脉球囊损伤后内膜增生

杨炯¹,王挺¹,杨怡²,李枚岭¹,王强¹,陈垦¹,杨大春³

1. 成都军区总医院心内科
2. 四川医科大学（忠山校区）
3. 成都军区总医院

收稿日期:2016-12-12 修回日期:2017-03-21 出版日期:2017-06-05 发布日期:2017-05-26
通讯作者: 杨大春 E-mail:yangdc71@126.com
基金资助:
国家自然科学基金

Over expression of TMEM66 attenuates the neointima formation of carotid artery after balloon injury in rats

Received:2016-12-12 Revised:2017-03-21 Online:2017-06-05 Published:2017-05-26
Supported by:
National Natural Science Foundation of China

摘要/Abstract

摘要： 目的：研究跨膜蛋白66（TMEM66）在大鼠颈动脉球囊损伤后内膜增生中的作用。方法:将SD大鼠随机分为对照组，左侧颈动脉球囊损伤组和TMEM66过表达组（n=10）。HE染色检测各组血管内膜增生情况。Western blot、q-PCR及IHC分别检测颈动脉血管中TMEM66的表达。CCK8和划痕实验分别检测TMEM66过表达后原代培养血管平滑肌细胞（VSMC）的增殖和迁移。结果：颈动脉球囊损伤血管中TMEM66的表达量较对照组显著下降(P＜0.05)。球囊损伤后血管内膜明显增生，慢病毒特异性转染TMEM66过表达可显著逆转血管内膜增生(P＜0.05)。上调TMEM66能够改善PDGF所诱导的VSMC增殖和迁移(P＜0.05)。结论：TMEM66可以改善大鼠颈动脉球囊损伤后内膜增生。

关键词: 跨膜蛋白66, 球囊损伤, 内膜增生

Abstract: Objective To clarify the role of transmembranprotein 66(TMEM66) in neointima formation of carotid artery induced by balloon injury in rats. Methods SD rats were randomly divided into three groups (n=10), including control group, the left carotid artery balloon injury group and injury group added with lentiviruses, respectively. Accordingly, the neointima formations of carotid arterywere detected by H/E staining;the expressions of TMEM66 in carotid arteryinjuried by balloon.were determined by western blot,q-PCRand immunohistochemistry, and the migration and proliferation of VSMCs were measured by scratch test and CCK8, respectively. Results Compared with control group, the expression of TMEM66 in carotid artery were obviously decreased after balloon injury (P＜0.05). Most importantly, the neointima formation of carotid artery was significantly reversed by overexpression of TMEM66 via specific lentiviruses (P＜0.05). Meanwhile, it is obviously that upregulation of TMEM66 can alleviate the migration and proliferation of VSMCs by PDGF(P＜0.05). Conclusions TMEM66 is able to attenuate the neointima formation of carotid artery after balloon injury.

Key words: TMEM66, balloon injury, neointima formation

中图分类号:

R543.5

杨炯王挺杨怡李枚岭王强陈垦杨大春. 跨膜蛋白66过表达减轻大鼠颈动脉球囊损伤后内膜增生[J]. 基础医学与临床, 2017, 37(6): 775-780.