关键词: 特异性作用于RNA的腺苷脱氨酶B1, 线粒体抗病毒信号蛋白，RNA编辑

Abstract: Objective To investigate the effect of ADAR2 on the expression of MAVS and its mechanism. Methods The RT-qRCR was used to detect the expression of ADAR gene family, the expression level of ADAR2 and MAVS in cells. The effect of ADAR2 on the 3 'UTR region of MAVS was detected by Pyrosequencing. To detect the expression of luciferase by dual luciferase reporter plasmid assay; The expression of ADAR2 and MAVS were detected by Western blot. Results In the ADAR family, the abundance of ADAR1 was the highest, followed by ADAR2, but the expression of ADAR3 was poor, which was almost impossible to detect（P < 0.05）. ADAR2 plays a critical role in RNA editing of chr20:3869744 sites on the 3 'UTR region of MAVS（P < 0.001）. On the 3 'UTR editing site of MAVS, the luciferase activity of the edited G allele was significantly lower than that of the normal A allele（P < 0.01）. At the level of transcription and translation, ADAR2 significantly inhibited the expression of MAVS（P < 0.05）. Conclusions ADAR2 by editing MAVS' 3 'UTR on the chr20:3869744 site, which makes the occurrence of A to G replacement, inhibits the expression of MAVS.

Key words: adenosine deaminase, RNA specific B1, mitochondrial antiviral signaling protein, RNA editing