关键词: 表皮生长因子, 雌激素受体, Ack1, 达沙替尼, 磷酸化, 子宫内膜癌

Abstract: Objective To investigate the effect of epidermal growth factor (EGF) on the proliferation of endometrial adenocarcinoma cells，phosphorylation of estrogen receptor α (ERα)and Ack1 in the absence of estrogen. Methods Ishikawa cell line was stimulated by EGF without estrogen settings, Cell Counting Kit-8 (CCK-8) was used to evaluate cell proliferation, Western blot was used to detect ER α phosphorylation and Ack1 phosphorylation. Giving tyrosine inhibitor dasatinib to assess the effect of EGF on cell proliferation，phosphorylation of ERα and Ack1 in Ishikawa cells.Results EGF enhanced the proliferation of endometrial adenocarcinoma cells (p<0.05). EGF induced ERα phosphorylation at Tyr-537 and phosphorylation of Ack1. Compared with untreated control, Dasatinib inhibited the proliferation of endometrial adenocarcinoma cells (p<0.05), phosphorylation of ERα Tyr-537 and Ack1. Conclusions EGF promoted Ishikawa cells proliferation in the possible way of activating ER α site-specific phosphorylation at Tyr-537 and phosphorylation Ack1, which could be blocked by dasatinib.

Key words: Epidermal growth factor, Estrogen receptor, Ack1, dasatinib, phosphorylation , endometrial cancer