关键词: UHRF2, 羟基脲, DNA损伤应答, 组蛋白H3K9乙酰化, 细胞增殖

Abstract: Objective To investigate the effect of UHRF2 on H3K9 acetylation and cell proliferation in DNA damage response induced by Hydroxyurea. Methods HEK293 cells cultured in vitro treated with Hydroxyurea for different time to construct cell models of DNA damage; HEK293 cells were transfected with pCMV-3×FLAG-UHRF2.We evaluated transfection efficiency of pCMV-3×FLAG-UHRF2 and H3 K9 acetylation in DNA damage response with Western blot. CCK8 assay was used to detect cell proliferation. Results HEK293 cells treated with 2.5mmol/L Hydroxyurea 12h later, obvious DNA damage was detected. After pCMV-3×FLAG-UHRF2 was transfected into the cells, The expression of UHRF2 was increased (P<0.01). The acetylation level of H3 K9 significantly increased (P<0.05). The cell proliferation was decreased By CCK8 (P<0.05). Conclusion UHRF2 can promote H3 K9 acetylation and cell proliferation in DNA damage response induced by Hydroxyurea.

Key words: UHRF2, Hydroxyurea, DNA damage response, H3K9 acetylation, cell proliferation