关键词: 低氧性肺动脉高压, c-Met蛋白, 内皮祖细胞, 细胞分布

Abstract: Objective To discuss overexpression c-Met protein mediated distribution of EPCs in HAPH model.Methods To establish the model of HPAH,primary culture rat bone marrow-derived EPCs and identificated, adenoviral with c-Met gene infected EPCs and Cells were divided into control group,viral load group,c-Met over-expression group.c-Met expression in protein and gene level were detected by Western Blot and Real time PCR , endothelial progenitor cells were marked by CM-Dil , the cells were infused through the tail vein of rat,distribution of the cells were observed in of the major organs afte 1W. Results c-Met mRNA expression of control group、viral load group and c-Met over-expression group were 0.19，0.17 and 1.85；c-Met protein expression were 0.24、0.17 and 0.82. The positive rate of EPCs in lungs、liver、spleen and kideny of EPCs group were 1.7 ％,0.5 ％,7.9 ％ and 9.4 ％；The positive rate of EPCs in viral load group were：1.5 ％,0.4 ％,8.3 ％ and 8.5 ％；The positive rate of EPCs in c-Met EPCs group were 9.3 ％,0.2 ％,1.3 ％ and 0.3 ％,The positive rate of EPCs in c-Met EPCs group significant higher in lung and decreased in spleen and kideny compared with control group（P＜0.001）.Conclusions Over expression of c-Met protein make EPCs superiority distributed in lung of HPAH model.

Key words: HPAH, c-Met protein, EPCs, cell distribution