病毒感染后RNF167的表达下调抑制小鼠腹腔巨噬细胞产生IFN-β

基础医学与临床 ›› 2016, Vol. 36 ›› Issue (1): 85-88.

病毒感染后RNF167的表达下调抑制小鼠腹腔巨噬细胞产生IFN-β

王知明,田雨

海军机关门诊部

收稿日期:2015-05-25 修回日期:2015-07-20 出版日期:2016-01-05 发布日期:2015-12-29
通讯作者: 王知明 E-mail:wzming@hotmail.com

Down-regulation of RNF167 suppresses the production of IFN-β in viral-infected mouse peritoneal macrophages

Received:2015-05-25 Revised:2015-07-20 Online:2016-01-05 Published:2015-12-29

摘要/Abstract

摘要： 目的探讨RNF167对病毒感染的小鼠腹腔巨噬细胞产生IFN-β的调控作用。方法 RNA病毒VSV和DNA病毒HSV-1感染小鼠腹腔巨噬细胞后，Western blot检测RNF167的表达水平。利用RNA干扰减少小鼠原代腹腔巨噬细胞中RNF167的表达后，病毒感染小鼠腹腔巨噬细胞，实时荧光定量PCR法检测IFN-β的mRNA水平，ELIAS法检测细胞上清中IFN-β的浓度。Western blot 法检测IFN-β的转录因子IRF3的磷酸化（p-IRF3）水平。结果 VSV感染巨噬细胞4和8 h后，RNF167表达水平显著下降 (P < 0.01)，而HSV-1感染后RNF167的表达没有显著变化。si-RNF167降低小鼠腹腔巨噬细胞RNF167表达水平后，与对照细胞相比，感染RNA病毒后细胞中IFN-β的mRNA水平及上清中IFN-β水平显著下降（P < 0.01），p-IRF3水平也显著下降。而感染HSV-1病毒后上述指标均无差异。结论 RNF167能够特异性地调控RNA病毒感染的巨噬细胞中IFN-β的表达。

关键词: RNF167, IFN-β, IRF3, 天然免疫

Abstract: Objective To investigate the regulation of IFN-β expression by RNF167 in viral-infeted macrophages. Methods The expression of RNF167 was detected by Western blot in mouse peritoneal macrophages infected with either RNA virus VSV or DNA virus HSV-1. The expression of IFN-β was determined by real-time quantitive PCR (q-PCR) and ELISA assay in macrophage transfected with si-RNF167 and viral infection. P-IRF3 level was analyzed by Western blot. Results RNF167 was down-regulated in VSV infected mouse peritoneal macrophages （P < 0.01）. Knockdown of RNF167 by si-RNF167 significantly decreased the expression of IFN-β and the level of p-IRF3 in RNA viral-infected cells （p < 0.01, but not in HSV-1-infected cells. Conclusion With RNA viral infection, the level of RNF167 expression was decreased, which specifically regulated IFN-β expression in mouse peritoneal macrophages.

Key words: RNF167, IFN-β, IRF3, innate immunity

王知明田雨. 病毒感染后RNF167的表达下调抑制小鼠腹腔巨噬细胞产生IFN-β [J]. 基础医学与临床, 2016, 36(1): 85-88.