关键词: 维生素E, H2O2, 抗凋亡信号传导, 抗氧化酶体系

Abstract: Objective:To explore the antioxidant effect of Vitamin E on both in the rat in vivo and in hepatic RBL cells in vitro.Methods:The cultured human hepatic RBL cells were exposed to H2O2 as an oxidant and treated with 0.4mM doze of Vitamin E before or after exposure of H2O2. The cell survival and apoptosis were detected by MTT and TUNEL assays. The expression level of NF-kB，Bcl-2，Bax，Hsp-70 and Caspase-3 was determined by immunohistochmistry and Western blotting. Will clean level only 20 male Wistar rats were divided into control group and the big and small doses of vitamins E35 and 15 mg/kg 2 ml lavage solution，1 times a day，a total of three times，the biochemical method to detect within the plasma after 3 and 6 d T – AOC，SOD，GSH and MDA levels. Results: H2O2 damage group (Ec) apoptosis rate increased (P< 0.01)，intracellular Bax，HSP – 70，the nf-kappa B and caspase 3 significantly higher (P< 0.01)，while the Bcl - 2 decreased significantly (P< 0.01)，vitamin E after the intervention can significantly alleviate the above changes (P< 0.01)，H2O2 damage before intervention is better than that after intervention. Lavage after 3 d plasma T - the AOC，SOD，higher level of GSH，MDA lowered (P< 0.01)，and 6 d after the relevant index change is more significant (P< 0.05). Conclusion: The results showed that the Vitamin E displays anti-oxidant capacity through upregulating the antioxidant enzyme system and regulating the expression of relevant proteins.

Key words: Vitamin E, hydrogen peroxide, anti-oxidant enzyme system, anti–apoptoic signal transduction