关键词: ZNF330, CD34+细胞, K562 细胞, 红系分化, ZNF408

Abstract: Objective To explore the effects of ZNF330 on erythroid differentiation of K562 cells and its mechanism. Methods Realtime PCR was performed to detect the expression of ZNF330 in K562 cells induced by hemin. After CD34+ cells were infected by the recombination lentivirus ZNF330-RNAi, Realtime PCR was applied to detect the expression of CD235a and γ-globin. The luciferase report assay was performed to examine if ZNF330 could act as a trans-acting factor in 293T/17 cells. Co-Immunoprecipitation (Co-IP) was applied in 293T/17cells to detect the interaction between ZNF330 and ZNF408 which was involved in mRNA degradation. Results The expression of ZNF330 was up-regulated after hemin treatment. The expression of CD235a and γ-globin was decreased after the express of ZNF330 was inhibited. Luciferase report assay showed that ZNF330 has no effect on report gene. Co-Ip in two ways confirmed the direct binding between ZNF330 and ZNF408.Conclusion ZNF330 can promote erythroid differentiation, and a possible mechanism is that ZNF330 inhibits the function of ZNF408, a factor that is involved in mRNA degradation, through the interaction between the two proteins.

Key words: ZNF330, CD34+细胞, K562 cells, erythroid differentiation, ZNF408