关键词: 胃肿瘤, Hippo/YAP, Wnt/β-catenin, 信号转导通路

Abstract: Objective To investigate the expressions and correlation of YAP andβ-catenin in the gastric carcinoma(GC) tissues and the paired normal gastric tissues. Methods Fluorescence quantitative PCR(RT-qPCR）, immunocytochemistry staining and Western blotting were used to detect the mRNA and protein expressions of YAP and β-catenin in GC tissues and the paired normal gastric tissues，The correlations between the expressions and the clinicopathological characteristics of GC were analyzed statistically. Results The positive expression of YAP and β-catenin were respectively 72% (36/50) and 64% (32/50), and the positive expression of paired normal gastric tissues were 14% (7/50) and 20% (10/50), P<0.05; The mRNA and protein levels of YAP and β-catenin in GC tissues were higher than those in the paired normal tissues（P<0.05).Furthermore, the expression was obviously correlated to cell differentiation, lymph node metastases and clinical stage of GC( P< 0.05). The significantly positive correlation was found between the expressions of YAP and β-catenin in GC tissues(r=0.72, P<0.05). Conclusion The high expression of YAP and β-catenin coexist in GC tissues. It may be related to malignant transformation and abnormal proliferation of cells, which facilitates the occurrence and development of GC.

Key words: stomach neoplasm, Yes-associated protein, β-catenin, signal transduction pathway