关键词: 重组新城疫病毒, RVG, 肺癌, 迁移, EMT

Abstract: Objective Observe the effects of rl-RVG on migration of lung adenocarcinoma A549cells, preliminarily explore potential mechanism. Methods The group infected with the rl-RVG was experimental group, The group infected with NDV and the group not infected with virous were control groups.The experimental detected for the expressions of RVG and NDV- HN proteins by Western blot．Cell growth experiment determined the best active concentrations of newcastle disease virus and rl-RVG；Observe the effects of rl-RVG and NDV on migration of lung adenocarcinoma A549 cells by scratch assay and Transwell method.The expression of E-cadherin and MMP2 was observed by Western blot and immunofluorescence.The LaSota strain of NDV was used as control group and PBS was the blank control.Results It Proved successful transfection,both RVG and NDV proteins didn’t expressed in blank control group．RVG protein expressed in rl-RVG group and NDV protein expressed in both rl-RVG group and NDV group. Cell proliferation was inhibited by more than 5×10-5 diluted concentrations in rl-RVG group and NDV group compared with the blank control group（P＜0.05). So the selected dilution of solubility was 10-6 rl-RVG and NDV. After infected with rl-RVG, migration distance and number of cells significantly reduced（P＜0.05). After A549 cells were infected with rl-RVG , the expression of E-cadherin was enhanced（P＜0.05)and the expression of MMP2 was weaken compared with the blank control group and NDV group（P＜0.05).Conclusion Recombinant avirulent newcastle disease virus can inhibit the migration of A549 cells in vitro, which may be attributed to regulatory factors of E-cadherin and MMP2 in the procession of epithelial mesenchymal transition EMT.

Key words: NDV, RVG, lung cancer, migration, EMT