关键词: 关键词：卵磷脂胆固醇酰基转移酶, DNA甲基化, 动脉粥样硬化, 血脂

Abstract: Objective To analysize the change of LCAT DNA methylation level and the relationship with blood lipids in Hui patients. Methods The 35 AS patients and 42 controls from Hui ethnic were confirmed by carotid artery Doppler ultrasound, LCAT mRNA expression were determined by real-time reverse transcription–polymerase chain reaction (qRT-PCR). The methylation status of LCAT gene promoter region was studied using the nest tounchdown methylation specific polymerase chain reaction (nt-MSP). The serum lipids were measured by ADVIA 2400 Automatic Biochemistry Analyzer. Results The TC,TG and LDL were higher than the normal group, HDL were lower than the normal group. Besides, compared with the normal group, the LCAT mRNA level of AS patients decreased by 61.2%(P＜0.01), and the LCAT DNA methylation level increased 1.3 times(P＜0.05). Conclusion The LCAT DNA hypermethylation is related to the occurrence and development of AS and the changes of serum lipids in Hui patients.

Key words: Key Words: lecithin cholesterol acyltransferase, DNA methylation, atherosclerosis, blood lipids