载有槲皮素的PEG-PE胶束对乳腺癌细胞耐药性的逆转效应

基础医学与临床 ›› 2015, Vol. 35 ›› Issue (2): 174-177.

载有槲皮素的PEG-PE胶束对乳腺癌细胞耐药性的逆转效应

吴金花¹,段金虹¹,许海燕¹,杨先达²

1. 中国医学科学院北京协和医学院基础医学研究所
2. 中国医学科学院基础医学研究所

收稿日期:2014-07-11 修回日期:2014-10-23 出版日期:2015-02-05 发布日期:2015-01-23
通讯作者: 杨先达 E-mail:ayangmd@gmail.com
基金资助:
科技部重大科学计划

Quercetin-loaded PEG-PE micelles reverse drug resistance of MCF-7 ADRr human breast cancer cells

Received:2014-07-11 Revised:2014-10-23 Online:2015-02-05 Published:2015-01-23
Contact: Xian-da YANG E-mail:ayangmd@gmail.com

摘要/Abstract

摘要： 目的探索载有槲皮素的PEG-PE聚合物胶束(M-Q)能否在体外逆转耐阿霉素人乳腺癌细胞（MCF-7 ADRr）的耐药性。方法将PEG-PE聚合物与槲皮素混合制备脂膜，加0.9%氯化钠注射液形成胶束，用动态光散射粒径仪表征其尺寸分布，并将其和阿霉素与MCF-7 ADRr细胞共孵育72h，用MTS方法检测对肿瘤细胞的杀伤率。结果 PEG-PE聚合物胶束能有效运载槲皮素，其包封率高达74%以上，平均粒径为11.11nm左右，与游离槲皮素相比，M-Q能在体外更大程度地逆转MCF-7 ADRr细胞对于阿霉素的耐药性（P<0.05），且PEG-PE胶束自身对靶细胞无明显杀伤作用。结论载有槲皮素的PEG-PE胶束在逆转肿瘤耐药性方面具有应用潜能。

关键词: PEG-PE, 胶束, 槲皮素, MCF-7 ADRr细胞

Abstract: Objective To explore whether quercetin-loaded PEG-PE micelles(M-Q) will synergize the growth-inhibitory activity of adriamycin(ADR)by reversing the drug resistance of MCF-7 ADRr breast cancer cells in vitro. Methods M-Q were formed by adding saline to lipid film containing quercetin and PEG-PE. The size of M-Q was characterized by dynamic light scattering(DLS). The inhibition of MCF-7 ADRr cells was evaluated by MTS assay after incubation with M-Q and ADR. Results The incorporation efficiency of quercetin by the micelles was above 74%.The average size of M-Q was 11.11nm. Compared with the quercetin dissolved in ethanol, M-Q more effectively reversed the drug resistance of MCF-7 ADRr cells in vitro. Conclusions PEG-PE micelles may have potential for delivering quercetin to cancer cells for reversal of drug resistance.

Key words: PEG-PE, micelles, quercetin, MCF-7 ADRr cells

吴金花段金虹许海燕杨先达. 载有槲皮素的PEG-PE胶束对乳腺癌细胞耐药性的逆转效应[J]. 基础医学与临床, 2015, 35(2): 174-177.