关键词: 【关键字】 SRPX2, 血管生成, 细胞外基质, 条件培养基

Abstract: Objective To evaluate the proangiogenesis ability of extracelluar matrix proteins SRPX2 on HUVECs. Methods pcDNA3.1-SRPX2 vector (transfection group) and pcDNA3.1 vector (negative group) were transfected into HEK293T cells, divided into 3 groups，including blank group. The proliferation of HUVECs (absorbance, A450) was detected by CCK-8 kit. The transmembrane cell number was counted by Transwell migration and wound healing assay to evaluate the migration ability of HUVECs．A three dimensional culture system of cells was constructed on the Matrigel, and tube formation number of HUVECs was assessed． Results The proliferation of HUVECs ( absorbance,A450 ) among transfection group，negative group and blank group had no significant difference (P>0.05). A signifiant difference was noted in the total branch point of capillary tubes among transfection group, negative group and blank group ([97±4]/field versus[57±3] and [54±3]/field) (P<0.05). In wound healing assay, the distance of transfection group compared to 0, 6 and 12 h were both significantly larger than that of negative group and blank group ([90±6], [37±7], [36±4] μm and [135±5], [65±8], [63±4] μm respectively, both (P<0.05)). In Tranwell assay, the number of migrating cells in the transfection group was significantly more than negative group and blank group ([549±10]/field vs [334±11]and [329±12]/field(P<0.05)) after co-culture 16 h. Conclusion SRPX2 may enhance angiogenesis ability by promoting the migration ability and tubing on the Matrigel of HUVECs.

Key words: [Key words] SRPX2, Angiogenesis, Extracellular matrix, Conditioned medium