环磷酰胺下调大鼠睾丸OCT-4和GDNF表达

基础医学与临床 ›› 2013, Vol. 33 ›› Issue (7): 824-828.

环磷酰胺下调大鼠睾丸OCT-4和GDNF表达

常青,杨震,王燕蓉,马文智,黑常春,沈新生

宁夏医科大学基础医学院

收稿日期:2012-11-19 修回日期:2013-01-30 出版日期:2013-07-05 发布日期:2013-06-26
通讯作者: 常青 E-mail:seryin55@163.com
基金资助:
促卵泡激素对睾丸支持细胞胶质细胞源性神经营养因子表达调节的机理研究;睾丸化疗损伤对精原干细胞增殖和分化影响的研究

Down regulation of the expressions of OCT-4 and GDNF by Cyclophosphamide in rat testis

Received:2012-11-19 Revised:2013-01-30 Online:2013-07-05 Published:2013-06-26
Contact: qing Chang E-mail:seryin55@163.com

摘要/Abstract

摘要： 目的探讨抗肿瘤药物环磷酰胺对大鼠睾丸和精原干细胞相关因子OCT-4和GDNF表达的影响。方法 12只8周龄大鼠随机分为对照组和实验组，每组6只；实验组每天腹腔注射环磷酰胺50mg/kg，连续3d，用药4周后PAS染色法观察大鼠睾丸组织学变化，精原细胞，生精小管直径和附睾尾部精子数量。免疫组织化学法检测精原干细胞相关分子OCT-4和GDNF表达水平。结果与对照组比较，实验组大鼠体重、睾丸和附睾重量均显著减轻，睾丸组织学无明显改变，附睾精子活动度降低，附睾精子和精原细胞数量减少。实验组GDNF表达明显减少，而OCT-4表达水平无明显变化。结论环磷酰胺短期用药可造成精子发生的损害，其中精原细胞的减少可能与损害后GDNF表达下调有关。

关键词: 环磷酰胺, 精子发生, OCT-4, GDNF

Abstract: Objective To observe the effects of cyclophosphamide on the expressions of GDNF and OCT-4 that related functions of spermatogonial stem cells in rats. Methods Twelve 8 weeks male SD rats were randomly divided into control and experimental group. The rats in experimental group were injected cyclophosphamide (50mg?kg-1?d-1) intraperitoneally for three days. The testis and epididymis were removed after 4 weeks of injection to assess the weight , sperm mobility, sperm and spermatogonia numbers and diameter of seminiferous tubule. The expressions of OCT-4 and GDNF were measured by immunohistochemistry assay. Results Compared with the control group, the weights of testis and epididymis were reduced. Although there was no observed changes of testicular histology, the epididymal sperm mobility, and the numbers of epididymal sperm and spermatogonia were reduced. The expression of GDNF was decreased and that of OCT-4 was not changed. Conclusion The spermatogenesis was damaged by short-time treatment of cyclophosphamide, it might be that cyclophosphamide caused the reduction of spermatogonia through the downregulation of GDNF.

Key words: Cyclophosphamide, spermatogenesis, OCT-4, GDNF

常青杨震王燕蓉马文智黑常春沈新生. 环磷酰胺下调大鼠睾丸OCT-4和GDNF表达[J]. 基础医学与临床, 2013, 33(7): 824-828.

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