关键词: 大肠癌, PDTC, 血管生成

Abstract: Objective To investigate the effect of NF-κB inhibitor PDTC on the expressions of angiogenic factors in colon carcinoma Lovo cells in vitro. Methods NF-κB inhibitor PDTC was used to treat colon carcinoma Lovo cells. Untreated Lovo cells were served as control group, Lovo cells that were treated with PDTC were served as experimental group. The expressions of NF-κB, p-IκBα, VEGF and b-FGF in each group were detected by Western blot. The ability of HUVEC migration was observed by Transwell migration assay. The supernatant of Lovo cells in each group was respectively collected and added into HUVEC culture medium separately, then CCK-8 was used to check the ability of HUVEC proliferation. Results Western blot results showed that the expressions of NF-κB, p-IκBα, VEGF and b-FGF in the experimental group were all obviously lower than that in the control group（P<0.05）. The results of migration assay showed that the quantity of migratory HUVEC through the microporous membrane was decreased in the experimental group than that in the control group（P<0.05）and migratory inhibition rate was 49.2%. The results of CCK-8 showed that the proliferation of HUVEC in the experimental group was obviously lower than that in the control group（P<0.05）, and the inhibition rate was 73.2%. Conclusion In Lovo cells，the NF-κB inhibitor PDTC may inhibit the ability of angiogenesis of Lovo cells in vitro through down-regulating the activity of NF-κB.

Key words: colon carcinoma, PDTC, angiogenesis