关键词: 雪旺细胞, 高糖, 氧化应激损伤, PARP-1, 筋脉通

Abstract: Objective To investigate the effects of medicated serum prepared by administration of Jinmaitong(JMT), a compound Chinese herbal medicine, on oxidative damage and poly ADP-ribose polymerase-1(PARP-1) of Schwann cells cultured in high-glucose medium, Method SD rats were divided into normal control group(distilled water),JMT(1.31g/(kg?d)) group and vitamin C(0.08g/(kg?d))group to prepare medicated serum. Bilateral sciatic nerves of new born SD rats were used to separate Schwann cells. Schwann cells cultured in high-glucose medium were divided into high glucose group (50mmol/L glucose medium，JMT group(JMT-medicated serum) and vitamin C(VC)group (VC-medicated serum).Schwann cells cultured in DMEM were used as the normal control. After 48h culturing, the level of ROS was measured by confocal laser scanning microscope with 2',7'-dichlorofluorescein(DCF) as a molecular probe and the expression of PARP-1 protein was detected by Western blot. Result: (1) Compared with high glucose group, the fluorescence intensities of ROS-DEC in Schwann cells cultured in JMT and VC groups were weaker significantly(P＜0.01). There were no significant differences between these two treated groups(P＞0.05). (2)Compared with high glucose group,the expression of PARP-1(89ku) in Schwann cells cultrued in JMT group decreased significantly(P＜0.01).The expression of JMT group was also much lower than that of VC group(P＜0.01).Conclusion: The medicated serum of JMT could down-regulate the expression of ROS and PARP-1 of Schwann cells cultured in high glucose medium, reduce the oxidative DNA damage.

Key words: Schwann cell, high glucose, oxidation damage, PARP-1, Jinmaitong capsule