TWEAK和Fn14在腰椎间盘退变髓核组织中的表达及意义

基础医学与临床 ›› 2012, Vol. 32 ›› Issue (9): 1053-1058.

TWEAK和Fn14在腰椎间盘退变髓核组织中的表达及意义

陈春永¹,沈正清²,叶君健¹

1. 福建医科大学附属第一医院
2. 福建医师大学附属第一医院

收稿日期:2010-10-21 修回日期:2011-12-08 出版日期:2012-09-05 发布日期:2012-08-28
通讯作者: 叶君健 E-mail:yejunjian@medmail.com.cn
基金资助:
福建省自然科学基金（C0710010），教授基金（JS09007）。;省自然科学基金

Expression and significance of TWEAK and Fn14 in the degenerated and traumatic lumbar discs

Received:2010-10-21 Revised:2011-12-08 Online:2012-09-05 Published:2012-08-28
Contact: YE Jun-Jian E-mail:yejunjian@medmail.com.cn

摘要/Abstract

摘要： 摘要：目的研究TWEAK和Fn14在腰椎间盘退变髓核组织中的表达及其临床意义。方法用半定量RT-PCR和免疫组织化学法检测实验组（30例退变腰椎间盘）和对照组（10例创伤腰椎间盘）中TWEAK和Fn14 mRNA和蛋白表达。结果实验组TWEAK mRNA表达显著高于对照组（0.949士0.093 VS 0.653士0.110，P＜0.01)，实验组TWEAK蛋白表达显著高于对照组（0.682士0.126 VS 0.397士0.057，P＜0.01)，实验组Fn14mRNA表达显著高于对照组(0.936士0.125 VS 0.632士0.059,P＜0.01)，Fn14蛋白表达显著高于对照组(0.540士0.051 VS 0.344士0.072,P＜0.01)。结论TWEAK和Fn14的表达增加可能参与腰椎间盘退变的进程。

关键词: 肿瘤坏死因子样凋亡微弱诱导剂, 成纤维细胞因子诱导14, 椎间盘, 椎间盘退变

Abstract: Abstract：Objective To investigate the expression of TWEAK and Fn14 in nucleus pulposus of human degenerated lumbar discs and evaluate their clinical significance. Methods The mRNA and protein expression of TWEAK and Fn14 in the experimental group（30 fresh pulpy nucleus of patients with lumbar disc herniation)and the control group(10 fresh pulpy nucleus of patients with traumatic lumbar vertebrae) were detected by semi-quantitative RT-PCR and immunohistochemical staining. Results The mRNA expression of TWEAK was significantly higher in the experimental group when compared with that of the control group( 0.949士0.0931 VS 0.653士0.110 ,P＜0.01); It was same to TWEAK protein (0.682士0.126 VS 0.397士0.057 ,P＜0.01); Fn14 mRNA (0.936士0.125 VS 0.632士0.059,P＜0.01); and Fn14 protein (0.540士0.051 VS 0.344士0.072 ,P＜0.01). Conclusions The increased expression of TWEAK and Fn14 in degenerate lumbar discs indicates that TWEAK and Fn14 may be involved in the process of lumbar intervertebral disc degeneration（IDD）.

Key words: tumor necrosis factor-like weak inducer of apoptosis, fibroblast growth factor-inducible 14, intervertebral disc

中图分类号:

R681.5

陈春永沈正清叶君健. TWEAK和Fn14在腰椎间盘退变髓核组织中的表达及意义 [J]. 基础医学与临床, 2012, 32(9): 1053-1058.