关键词: 关键词：肝纤维化, 腺病毒, 肝细胞生长因子, 白细胞介素10, 基因治疗

Abstract: Abstract：Objective To investigate the therapeutic effect of the recombinant adenovirus vector carrying hepatocyte growth factor (HGF) and human interleukin-10 (hIL10), Ad-HGF-hIL10, on CCl4-induced hepatic fibrosis in rats and to provide experiment data for hepatic fibrosis gene therapy. Methods Hepatic fibrosis models were established by CCl4 in SD rats, and a total of 58 rats were randomly divided into 4 groups. Ad-HGF-hIL10, Ad-HGF and Ad-hIL10 were injected into the tail vein of three testing groups respectively. Control group were injected with physiological saline. On the 15th day, blood samples were obtained from the rat hearts, and the serum TBIL, ALT, AST, TP and ALB levels were measured. After the rats were sacrificed, liver biopsies were performed and the liver tissues obtained were examined by light microscope. Results Compared with the control group, liver function of rats in the therapy group was obviously improved, while there is no obvious fibroplasia in the portal area. The ALT and AST level of the Ad-HGF-hIL10 therapy group were much lower than that of the Ad-HGF and Ad-hIL10 therapy group (P<0.01), while the A/G value of the Ad-HGF-hIL10 therapy group is more higher than the Ad-HGF and Ad-hIL10 therapy groups（P <0.01）. Conclusion：The double-gene therapy group has better therapeutic effect than other single-gene therapy groups. It was showed that HGF and IL-10 play a synergistic effect during hepatic fibrosis improvement. The experiment provides data for hepatic fibrosis gene therapy.

Key words: Key words：hepatic fibrosis, adenovirus, hepatocyte growth factor, interleukin-10, gene therapy