关键词: 幽门螺杆菌, 自噬, 人胃上皮细胞

Abstract: Abstract:Objective Make an observation of the autophagy induced by H. pylori infection in human gastric epithelium（GES-1）Methods Optimal multiplicity of infection (m.o.i) of H .pylori was determined by neutral red uptake method (NRU). GES-1 was infected by H. pylori with different time. Autophagy induced by H. pylori was interfered with 3MA, E64d and pepstatin A. Western blot was utilized for detecting the level of LC3 conversion and p62, which indicated the autophagy induction. The formations of autophagosomes were observed via immunofluorescence Results The optimum m.o.i was 400 for H. pylori (22695) infecting GES-1. At 2h p.i, autophagy was detected. It reached a peak level at 8h p.i, while it returned to the basic level at 24h p.i. 3MA was able to inhibit autophagy with obviously decrease of autophagosome formation. And E64d and pepstatin A could block autophagy downstream to some extent Conclusion H. pylori is capable of inducing autophagy in human gastric epithelium (GES-1), which can be regulated by autophagic inhibitors.

Key words: Helicobacter pylori, Autophagy, Human gastric epithelium