基础医学与临床 ›› 2012, Vol. 32 ›› Issue (2): 233-236.

• 短篇综述 • 上一篇    下一篇

四环素基因调控系统的研究进展

宫秀群1,马敏敏1,徐格林2   

  1. 1. 南京军区南京总医院
    2. 南京军区南京总医院神经内科
  • 收稿日期:2011-03-03 修回日期:2011-06-08 出版日期:2012-02-05 发布日期:2012-01-12
  • 通讯作者: 徐格林 E-mail:gelinxu@gmail.com
  • 基金资助:
    国家重点基础研究发展计划;南京军区南京总医院医学科研课题

New Strategies in Tetracycline-regulatable System

  • Received:2011-03-03 Revised:2011-06-08 Online:2012-02-05 Published:2012-01-12

摘要: 大量临床前研究均证实了病毒作为治疗基因载体的有效性和可行性。但将病毒载体技术用于临床疾病的治疗还面临一些挑战。其中,如何对病毒携带基因的表达实施定时定量调控是必需解决的一个关键问题。目前常采用的方法是在病毒载体基因组中构建调控系统中,而四环素基因调控系统(Tet系统)是迄今为止基因治疗研究中使用最广泛的系统。该系统通过改变培养基、体内四环素或其衍生物(如强力霉素)的浓度,诱导或抑制目标基因的表达。本文简要综述了Tet系统的研究进程及其在不同疾病中的应用。

关键词: 基因调控, 四环素基因调控系统, 基因治疗, 病毒载体

Abstract: Preclinical studies have confirmed the efficacy of viral vectors as a gene delivery tool,and recent clinical trials have shown promising results with this strategy. But before the clinical application,viral gene vectors should be reconstructed to make it can be regulated in a time- and dose-dependend fashion. Usually,a gene regulation system will be inserted into the vector genome. The tetracycline regulatory system (Tet system) is by far the most frequently used in gene therapy. By adjusting tetracycline or its derivatives (such as doxycyline) in medium or in vivo,the expression of target gene will be switched on or off. The development of Tet system and its application is discussed in this review.

Key words: Gene regulation, Tet system, Gene therapy, Viral vector

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