关键词: 结肠肿瘤, 细丝蛋白A, 侵袭, 转移

Abstract: Abstract:Objective To explore the inhibitory effect of Filamin A(FLNa) on invasion and metastasis of human colon carcinoma cell line SW480 and investigate its mechanisms of the action.Methods Expressing vector pcDNA3.1/V5-His-TOPO/FLNa was transfected into SW480 cells by lipofectamine protocols.After G418 selection,the cells SW480/FLNa expressing FLNa stably were obtained.Expression of FLNa in tumor cells was confirmed by reverse transcription polymerase chain reaction(RT-PCR) and Western blot.RT-PCR assay and Western blot was also employed to detect the expression of matrix metalloproteinases-9（MMP-9）.Cell proliferation in vitro were assessed by MTT assay.Inhibitory effect of filamin A on SW480 cells were observed by Wound healing assay,Transwell chamber experiment,and Matrigel invasion assay.Results The results of RT-PCR and Western blot demonstrated that the recombinant vector was correctly transfected into SW480 cells by liposome.The expression of MMP-9 mRNA and protein was lower in the SW480/FLNa cells than that in the wild SW480 cells；The inhibitory effect of FLNa on invasion and metastasis of SW480 cells were proved by wound healing assay,Transwell chamber experiment and matrigel invasion assay.Colusion FLNa has significant inhibitory effect on the invasion and metastasis of human colon carcinoma cell line SW480.The mechanism of action may be ralated with the suppressive effect of MMP-9.