关键词: 人脂肪间充质干细胞, 聚ε-己内酯, 内皮细胞

Abstract: Objective to study on adhesion ability, growth ability and differentiation into endothelial cells of human adipose-derived mesenchymal stem cells (hADSCs) planted on poly-ε-caprolactone material. Methods we choose the poly-ε-caprolactone with pore diameters of 60~80μm(called “small pore-size material” for short) and 180~200μm(called “large pore-size material” for short). Absorption and degradation rate were calculated on the 14th days. After hADSCs implantation in the materials with different diameters, comparison of seeding rate and growth were carried out by immunofluorescence with DAPI and scanning electron microscope (SEM). CCK-8 method was used to draw the growth curve. Endothelial differentiation with 40ng/ml VEGF and 10ng/ml bFGF after 50 days, vWF and VE-cadherin were detected by flow cytometers and Flk-1 was observed by immunofluorescence way. Results Absorption rates of small and large pore-size material on the 14th day were respectively calculated as 200% and 216.7%, while degradation rates were 13.3% and 21.7%. After implantation of hADSCs, cells looked long fusiform attaching on the material by SEM. Seeding rate of small pore- size material was 98.3%±0.3%, which was comparatively more than seeding rate as 90.3%±1.5% of large pore-size material, and growth curve observed the same. Hence, endothelial differentiation was carried out with small pore-size material. Differentiating after 50 days, positive ration of vWF was 80.9%±0.9% and VE-cadherin was 84.3%±1.1%. Flk-1 expression was observed in most of cells via immuofluorescence. Conclusion Poly-ε-caprolactone material with pore diameter of 60~80 μm is suitable for the growth of hADSCs which are also successfully differentiated into endothelial cells on it.

Key words: hADSCs, poly-ε-caprolactone, endothelial cells