关键词: 心肌肥厚, 转化生长因子β, 苯那普利, 坎地沙坦, 自发性高血压大鼠

Abstract: Objective To investigate the effects of benazepril and candesartan on hemodynamics parameters and transforming growth factor β1(TGF-β1) expression during myocardial hypertrophy in spontaneous hypertensive rats (SHR). Methods 12-week-old SHRs were treated with benazepril at 10 mg/(kg?d), candesartan at 4 mg/(kg?d) and a combination of 2 drugs at half dose, respectively, for 12 weeks. After 12weeks’ treatment, ejection fraction(EF) was acquired with the echocardiographic system. Left ventricular catheter was placed via right common carotid artery and hemodynamic parameters were determined. Left ventricular mass index(LVMI) and thickness of left ventricular posterior wall(LVPW) were also examined. Meanwhile, myocardium pathological structures were accessed with HE-staining. Expressions of TGF-β1 were detected using immunohistochemistry and western-blot analysis respectively. Results LVMI, thickness of LVPW, ratio of media area to lumen area in myocardial arteriole, expressions of TGF-β1 were decreased after the treatment of benazepril or candesartan (P<0.01). Hemodynamic parameters were also improved . After the combined treatment, the synergistic effect could be observed and EF were increased. Conclusion Synergistic attenuation of myocardial hypertrophy and improving of hemodynamic parameters in SHRs is produced by combined use of benazepril and candesartan possibly through negative modulation of TGF-β1 expression.