奥帕曲拉对内皮素-1诱导的大鼠心肌细胞肥大的影响

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1392-1393.

奥帕曲拉对内皮素-1诱导的大鼠心肌细胞肥大的影响

郑亚萍¹,韩坤²,蔡智慧¹

1. 漯河医学高等专科学校
2. 郑州大学基础医学院生理学教研室

收稿日期:2010-10-25 修回日期:2011-04-12 出版日期:2011-12-05 发布日期:2011-12-05
通讯作者: 郑亚萍 E-mail:zhengya85@sohu.com.cn
基金资助:
省卫生厅科研基金

The effect of omapatrilat on endothelin-1-induced cardiomyocyte hypertrophy in neonatal rats

Received:2010-10-25 Revised:2011-04-12 Online:2011-12-05 Published:2011-12-05

摘要/Abstract

摘要： 摘要：目的探讨奥帕曲拉（OMA）对内皮素-1（ET-1）诱导的大鼠心肌细胞肥大的作用及其机制。方法经差速贴壁法获得乳鼠心肌细胞，随机分为对照组、ET-1组、OMA 组、ET-1＋OMA组、ET-1＋OMA＋L-NAME组和ET-1＋OMA＋HS-142-1（GC受体阻断剂）组。用分光光度法测定NOS活性, 放射免疫法测定细胞内cGMP水平；液体闪烁计数仪测定3H-亮氨酸（[3H]-leu）掺入率及蛋白激酶C (PKC)活性；用HJ2000图像分析系统测定细胞表面积。结果10-6mol/L ET-1能显著增加心肌细胞表面积、PKC活性及[3H]-leu 掺入率, 降低NOS活性及 cGMP含量 (均P < 0.01); 10-7 mol/L OMA能显著抑制ET-1的上述作用（均P < 0.01），L-NAME、HS-142-1可部分阻断OMA的作用。结论OMA能抑制ET-1诱导的心肌细胞肥大效应，该作用部分通过NO/cGMP、PKC调控。

Abstract: Abstract：Aim To investigate omapatrilat on endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy in neonatal rats. Methods Isolated and cultured cardiomyocyte of neonatal Sprague-Danley (SD) rats were randomly divided into 6 groups: control, ET-1, OMA, ET-1＋OMA,ET-1＋OMA＋L-NAME and ET-1＋OMA＋HS-142-1. Nitric Oxide synthetase (NOS) activity was measured by Spectrophotometry. Intracellular cyclic GMP (cGMP) were measured by radioimmunoassay. [3H]-leu incorporation and PKC activity were evaluated by scintillation. Results Compared with the control group, 10-6mol/L ET-1 significantly increased the surface area, the activity of PKC and [3H]-leu incorporation of cardiomyocyte, decreased the activity of NOS and cGMP content (P < 0.01 for all); 10-7 mol/L OMA inhibited the above effects of ET-1 on cardiomyocyte (P < 0.01 v.s ET-1). L-NAME, HS-142-1 partially blocked the inhibitory effects of OMA ( P < 0.05). Conclusion The NO/cGMP and PKC may partially mediate the antiproliferation action of OMA in ET-1-induced cardiomyocyte hypertrophy.

中图分类号:

郑亚萍韩坤蔡智慧. 奥帕曲拉对内皮素-1诱导的大鼠心肌细胞肥大的影响 [J]. 基础医学与临床, 2011, 31(12): 1392-1393.