基础医学与临床 ›› 2011, Vol. 31 ›› Issue (12): 1383-1386.

• 研究论文 • 上一篇    下一篇

Annexin Ⅴ在缺氧滋养细胞中的表达及意义

辛虹,王惠兰   

  1. 河北医科大学第二医院妇产科
  • 收稿日期:2010-12-10 修回日期:2011-06-09 出版日期:2011-12-05 发布日期:2011-12-05
  • 通讯作者: 王惠兰 E-mail:wanghuilan20002000@yahoo.com.cn

The expression of Annexin Ⅴ in hypoxic trophoblast cells

Hong XIN1,   

  • Received:2010-12-10 Revised:2011-06-09 Online:2011-12-05 Published:2011-12-05

摘要: 目的 探讨膜联蛋白Ⅴ(AnnexinⅤ)在体内、外缺氧滋养细胞中的表达及其意义。方法:采用缺氧细胞培养、免疫组化、流式细胞术等实验技术测定缺氧滋养细胞内膜联蛋白Ⅴ的表达及变化。结果 (1)体外缺氧滋养细胞培养发现,AnnexinⅤ表达下降。(2)体内高血压缺氧状态下滋养细胞AnnexinⅤ的表达显著低于正常胎盘滋养细胞。(3)随着AnnexinⅤ浓度的增加,其抗凝功能呈梯度性变化。结论 缺氧滋养细胞中AnnexinⅤ表达呈明显下调,可能影响胎盘滋养细胞的生理功能,导致多种不良妊娠结局。

关键词: 缺氧, AnnexinⅤ, 凝血, 滋养细胞

Abstract: Objective To detect the expression of Annexin Ⅴ in hypoxic trophoblast cells including in vivo and in vitro and explore the signifiance. Methods The expression of annexin Ⅴin hypoxic trophoblast cells was detected by hypoxic cell culture, immunohistochemistry method, flow cytometry, and its effects on blood coagulation. Results (1) The expression of AnnexinⅤ decreased in trophoblast cell culture in vitro in hypoxia. (2) The expression of AnnexinⅤ also decreased in trophoblast cells in vivo in hypoxia (preeclampsia placenta).(3) With the increase of the concentration of Annexin Ⅴ, peripheral coagulation markers were observed clinical variation in vitro. Conclusion The expression of Annexin Ⅴin trophoblast cells in hypoxic was significantly reduced, which may affect placental function, and lead to a variety of adverse pregnancy outcomes.

Key words: hypoxia, Annexin Ⅴ, coagulation, trophoblast cells

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