关键词: 念珠菌病, 优势基因型, 单链构象多态性, 基因扫描

Abstract: Objective To explore the factors related to the recurrence of RVVC．Methods Samples were collected from the discharge of vagina，rectum，oral cavity in 55 patients and the discharge of balanus in 45 sexual partners of patients．Using SSCP analysis of internal transcribed spacer (ITS) region and sequence analysis of the 26S rDNA D1/D2 domain to identify the species of C. albicans of the samples. Genotypes of C. albicans strains were determined with use of SSCP combined to GeneScan analysis of C. albicans microsatellite locus I．Results The positive rates of vagina , oral and rectal Candida species colonization in the 55 women and 45 men were 100.0%(55/55)、61.8%(34/55)、1.8%(1/55) and 60.0%(27/45)，respectively．And the percentage of Candida strains among the vagina , rectum and balanus were concurrent．In the same person，the coincidence of Candida albicans genotype between the discharge from vagina and rectum were 86.2%．Anymore，54.5% of the Candida albicans genotype of the discharge from the vagina of RVVC patients were same as the discharge from the balanus of her sexual partner．Conclusion The state of having candida on the rectum or the balanus of sexual partner could be one of the factors of RVVC．