基础医学与临床 ›› 2011, Vol. 31 ›› Issue (1): 1-5.

• 研究论文 •    下一篇

经颅重频磁刺激增强大鼠大脑中动脉梗塞再灌注损伤后神经重塑

徐涛,邢岩,崔丽英   

  1. 北京协和医院
  • 收稿日期:2008-08-06 修回日期:2010-05-05 出版日期:2011-01-05 发布日期:2011-01-05
  • 通讯作者: 崔丽英

Effect of repetitive transcranial agnetic stimulation on the brain plasticity of rats after middle cerebral artery occlusion reperfusion injury

XU Tao 1,XING Yan 2,CUI Li-ying 1   

  1. 1. PUMC Hospital, CAMS & PUMC2.
  • Received:2008-08-06 Revised:2010-05-05 Online:2011-01-05 Published:2011-01-05
  • Contact: CUI Li-ying

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摘要： 目的 通过观察大鼠大脑中动脉缺血再灌注损伤后7d，大鼠神经功能、生长相关蛋白-43(GAP-43)、突触素(SYN)表达的变化，探讨经颅重频磁刺激（rTMS）对缺血性损伤后脑组织可塑性的影响。方法 制作大鼠右侧大脑中动脉梗塞再灌注模型，连续7d给予不同频率和强度的rTMS，同时设立假刺激组和假手术组进行对照。观察大鼠神经功能的变化，并应用免疫组化和Western blot技术检测脑组织GAP-43、SYN的表达。结果 rTMS组大鼠神经功能缺损评分较对照组明显降低，病灶侧脑组织内GAP-43、SYN蛋白水平较对照组显著增高。高频高强rTMS组与其他组差异显著(P<0.05)。结论 连续rTMS可以增强神经重塑作用，促进神经功能恢复。

关键词: 经颅重频磁刺激, 可塑性, 生长相关蛋白－43, 突触素

Abstract: Objective To explore the effect of rTMS on the plasticity of the brain after ischemic injury. Methods SD rat MCAO-reperfusion model was established and then were divided into four groups. Different rTMS were applied within 7 days after the reperfusion. The neurological impairment evaluation score of rats were recorded during this period. Immunohistochemistry and Western blot techniques were used to detect GAP-43 and SYN protein expressions. Results rTMS could improve rehabilitation of neurofunction. rTMS could increase the protein expression levels of GAP-43 and SYN. The therapeutic effect of high frequency and high intensity of rTMS was statistically better than other groups (P<0.05). Conclusion The application of rTMS continuously could enhance the plasticity of the brain and improve neurofunction.


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