关键词: PARG-shRNA, 基因沉默, 慢病毒, 大肠癌, 侵袭

Abstract: Objective To approach the effects of Poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion, migration and invasion. Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected. Western blot was used to test the expression of PARG, Poly(ADP-ribose) polymerase(PARP) and NF-κB. The lovo cell matrix adhesion, migration and invasion potencies were observed by cell matrix adhesion, migration and invasion assay. Results We obtained lovo cell strain with PARG gene silencing perpetually. The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (p<0.05, p<0.05). PARG gene silencing of lovo cells decreased the cell matrix adhesion, migration and invasion potencies. The inhibitory rates of lovo cell matrix adhesion, migration and invasion were 25.22%、38.71%、35.29% separately. Conclusion The data suggested that PARG gene silencing of lovo cells could inhibite the cell matrix adhesion, migration and invasion potencies, it probably related that PARG gene silencing decreased the activity of PARP and NF-κB. PARG probably plays an important role in the infiltration and metastasis of tumour.

Key words: PARG-shRNA, gene silencing, lentivirus, colon carcinoma, invasion