Abstract: Objective Through interfering the expression of Surviving with short hairpin RNA (shRNA) technology, to investigate the effect of down regulation of SURVIVIN on cell and subsequent apoptosis in cervical cancer cells Hela. Methods 1.The U6 promoter was obtained by PCR from liver of mouse and ligated into TA vector. RNAi vector(psSURVIVIN) and psN(control vector) containing of the U6 promoter was Established. 2. Using Hela cells as a model system, two groups were set up stably transfected with RNAi control plamid and psSURVIVIN (SURVIVIN RNAi plasmid), respectively. The expression of SURVIVIN in Hela cells was measured by using western-blotting and immunofluorescence methods. 3. The activity of caspase 3/7 was detected using Caspase-Glo(tm) 3/7 Assay Reagent.Results 1.Cell apoptotic rate was significantly increased by transfection with RNAi targeting plasmid, and cell was arrested at G0/G1, (P<0.01) ; 2. SURVIVIN expression was significantly decreased by transfection with RNAi targeting plasmid; the expression proportion was reduced by nearly 70%.( P<0.01); 3.The activity of caspase 3/7 of psSURVIVIN group obviously increased ( P<0.01).Conclusion These results showed that by inducing RNAi-targeting plasmid, SURVIVIN expression could be effectively decreased,moreover,the activity of caspase 3/7 of psSURVIVIN group obviously increased.