卵巢癌细胞株中c-Jun激活蛋白-1与p27kip1表达的相关性

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (9): 955-960.

卵巢癌细胞株中c-Jun激活蛋白-1与p27kip1表达的相关性

朱维培张弘钱志红刘利芬

苏州大学附属第二医院

收稿日期:2008-04-03 修回日期:2008-11-03 出版日期:2009-09-20 发布日期:2009-09-20

Correlation between c-Jun activation domain binding protein 1 and p27kip1 in ovarian cancer cell HO-8910

Wei-pei ZHU, Hong ZHANG, Zhi-hong QIAN, Li-fen LIU

the 2nd Hospital Affiliated to Suzhou University

Received:2008-04-03 Revised:2008-11-03 Online:2009-09-20 Published:2009-09-20

摘要/Abstract

摘要： 目的探讨c-Jun激活区结合蛋白-1（Jab1）与p27kip1在卵巢癌细胞株HO-8910中的表达变化及相互关系。方法用血清饥饿及饥饿后释放处理HO-8910细胞，分别用Western blot及核浆分离技术检测Jab1 、p27kip1表达及亚细胞定位；用免疫沉淀方法检测Jab1与p27kip1的结合情况；脂质体介导法将Jab1基因转染HO-8910细胞，Western blot及核浆分离检测p27kip1表达。结果血清饥饿导致HO-8910细胞生长周期停滞，p27kip1蛋白总量增加，且主要集中于胞核分布；Jab1蛋白总量减少,主要表达于核浆；血清释放后两者呈现相反的表达变化，但是p27kip1主要集中在胞质分布；p27kip1与Jab1在HO-8910细胞中存在相互结合的情况。脂质体转染Jab1后，p27kip1表达下降且定位有明显的胞质改变。结论 Jab1可能通过与p27kip1结合来介导p27kip1出核并影响其表达，从而参与调控HO-8910细胞的生长。

关键词: Jab1, p27kip1, 卵巢癌

Abstract: Objective To investigate the expression and relationship of Jab1 and p27kip1 in ovarian cancer cell HO-8910. Methods Cell were treated with serum starvation and release. The expression and distribution of Jab1 and p27kip1 were detected by western blot and subcellular fractionation. HO-8910 cells were transfected in vitro with pcDNA3.1-myc-Jab1. Western blot as well as subcellular fractionation was used to detect the expression and localization of p27kip1. Results: The growth of HO-8910 cells was blocked by serum starvation. The amount of p27kip1 increased while the amount of Jab1 decreased. The reverse changes happened after serum release. p27kip1 and Jab1 could form compound in HO-8910 cells detected by immunoprecipitation. 48h after transfected by pcDNA3.1-myc-Jab1 , the expression of p27kip1 decreased and the distribution of p27kip1 translocated from nucleus into cytoplasma in HO-8910. Conclusion: Jab1 may influence the location and expression of p27kip1 through integrating with p27kip1, and then may participate in regulating the growth of ovarian cancer cells through interfering with the function of p27kip1.

Key words: Jab1, p27kip1, ovarian cancer

朱维培张弘钱志红刘利芬. 卵巢癌细胞株中c-Jun激活蛋白-1与p27kip1表达的相关性[J]. 基础医学与临床, 2009, 29(9): 955-960.

Wei-pei ZHU; Hong ZHANG; Zhi-hong QIAN; Li-fen LIU. Correlation between c-Jun activation domain binding protein 1 and p27kip1 in ovarian cancer cell HO-8910[J]. Basic & Clinical Medicine, 2009, 29(9): 955-960.

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