二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (9): 938-942.

二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞

王海萍张雷邵素霞赵静

河北北方学院河北医科大学组织学胚胎学教研室

收稿日期:2008-09-03 修回日期:2008-11-17 出版日期:2009-09-20 发布日期:2009-09-20

Dimethyl sulfoxide induced bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro

Hai-ping WANG, Lei ZHANG, Su-xia SHAO, Jing ZHAO

Hebei North University

Received:2008-09-03 Revised:2008-11-17 Online:2009-09-20 Published:2009-09-20

摘要/Abstract

摘要： 目的探索二甲基亚砜（DMSO）体外诱导大鼠骨髓间充质干细胞（MSCs）向心肌样细胞分化的方法。方法取SD大鼠四肢骨骨髓，分离培养MSCs，应用终浓度为1.0%的DMSO定向诱导，相差显微镜观察细胞形态学变化，应用免疫细胞化学、激光扫描共聚焦显微镜技术检测结蛋白（desmin），α-横纹肌肌动蛋白（α-sarcomeric actin）、心肌特异性肌钙蛋白（C-TnT）的表达，透射电镜观察超微结构。结果 MSCs经体外诱导后分化的细胞均阳性表达desmin、α-sarcomeric actin、C-TnT。透射电镜下可见到平行排列的肌丝和丰富的粗面内质网。结论大鼠骨髓间充质干细胞体外在DMSO诱导下可以定向分化为心肌样细胞。

关键词: 骨髓间充质干细胞, 二甲基亚砜, 心肌细胞, 大鼠

Abstract: Objective To explore the optimal condition for the differentiation of rat bone marrow mesenchymal stem cells(MSCs) into cardiomyocyte-like cells in vitro by using DMSO.Methods SD rat MSCs were isolated from rat bone marrow and cultured, then induced by 1.0%DMSO for 24 h. The cultured cells were observed under phase-contrast microscope. The immunohistochemical technique and laser scanning confocal microscope (LSCM) were used for detecting the expression of desmin, α-sarcomeric actin and C-TnT. The ultrastructures were viewed with transmission electron microscopy.Results MSCs induced by DMSO could be identified by the positive staining for Desmin, α-Sarcomeric Actin and C-TnT.The number of cells stained positively in the 1.0% was visually larger than that of other groups. Transmission electron microscope showed that paralleled myofilaments and a lot of rough endoplasmic reticulum were formed. Conclusion MSCs could be induced to differentiate into cardiomyocyte-like cells by DMSO.

Key words: marrow mesenchymal stem cells, dimethyl sulfoxide, cardiomyocytes, Rat

王海萍张雷邵素霞赵静. 二甲基亚砜体外诱导大鼠骨髓间充质干细胞分化为心肌样细胞[J]. 基础医学与临床, 2009, 29(9): 938-942.

Hai-ping WANG; Lei ZHANG; Su-xia SHAO; Jing ZHAO. Dimethyl sulfoxide induced bone marrow mesenchymal stem cells differentiate into cardiomyocyte-like cells in vitro[J]. Basic & Clinical Medicine, 2009, 29(9): 938-942.

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