基础医学与临床 ›› 2009, Vol. 29 ›› Issue (10): 1050-1053.

• 研究论文 • 上一篇    下一篇

骨髓间充质干细胞移植对大鼠心梗后细胞因子VEGF、SDF-1、TGF-β1及心脏功能的影响

王挹青 李远鹏 侯炳波 郭晋村 张鹏   

  1. 福建医科大学教学医院厦门中山医院 福建医科大学教学医院厦门中山医院 福建医科大学教学医院厦门中山医院
  • 收稿日期:2008-10-17 修回日期:2009-02-12 出版日期:2009-10-20 发布日期:2009-10-20
  • 通讯作者: 王挹青

The effects of bone marrow mesenchymal stem cells administration on expression of VEGF、SDF-1、TGF-β1 and heart function in myocardial infarcts rats

Yi-qing WANG, Yuan-peng LI, Bing-bo HOU, Jin-cun GUO, Peng ZHANG   

  1. Fujian Medicine University Teaching Hospital Xiamen Zhongshan Hospital Fujian Medicine University Teaching Hospital Xiamen Zhongshan Hospital
  • Received:2008-10-17 Revised:2009-02-12 Online:2009-10-20 Published:2009-10-20
  • Contact: Yi-qing WANG,

摘要: 目的 观察急性心肌梗死大鼠移植异体骨髓间充质干细胞后血管内皮生长因子(VEGF)、基质细胞源性因子-1(SDF-1)、转化生长因子β1(TGF-β1)及心脏功能的变化。方法 密度梯度离心法和贴壁筛选法获得SD大鼠骨髓间充质干细胞,实验动物随机分为2组:细胞移植组即实验组(n=8)、无细胞移植组即对照组(n=8)。于移植4周后处死动物,免疫组化检测VEGF、SDF-1、TGF-β1及Buxco 系统测大鼠的心功能。结果 实验组在移植4周后免疫组化显示VEGF表达较对照组明显升高(15.02±1.87 VS 5.45±0.90,P<0.05), SDF-1较对照组明显升高(20.02±3.87 VS 8.24±1.17, P<0.01)、TGF-β1较对照组降低(13.24±2.07 VS 26.33±4.17,P<0.01);心脏功能亦较对照组显著改善(P<0.05)。结论 骨髓间充质干细胞移植到梗死心肌后可以干预VEGF、SDF-1、TGF-β1的分泌促进梗死后心脏功能的恢复。

关键词: 干细胞, 细胞因子, 心肌梗死, 细胞移植

Abstract: Objective To investigate the effects of bone marrow mesenchymal stem cells administration on expression of VEGF、SDF-1、TGF-β1 and heart function in myocardial infarcts rats. Methods MSCs were harvested from SD rats, collected from bone marrow, cultured and passaged in vitro, then purified by density gradient centrifugation and adhesive-screening method. Rats were randomly divided into 2 groups. In the control group (n =8) were not treated with stem cells as the experiment group, but saline of the same volume. Four weeks after the injection,the expression of VEGF、SDF-1、TGF-β1 and heart function were examined. Results Four weeks after the transplantation,Immunohistochemistry showed expression of VEGF and SDF-1 increased markedly in experiment group,and expression of TGF-β1 decreased. Heart function was improved in the experiment group(P<0.05). Conclusion MSCs transplantation can interfere in the secretion of VEGF、SDF-1、TGF-β1 and improve the heart function.

Key words: stem cells, cytokine, myocardial infarcts, transplantation

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