Abstract: Objective  To observe the effect of Yangxueqingnao granules on c-Fos protein expression in trigeminal nucleus caudalis (TNC) of nitroglycerin-induced migraine rats.  Methods  A total of 108 healthy male Wistar rats were randomly divided into normal control group, model group, high-dose treatment group, low-dose treatment group, high-dose prevention and low-dose prevention group, and then each group was randomly divided into 3 subgroups: 30, 60 and 180 min group. The experimental migraine rat model was established by subcutaneous injection of nitroglycerin. The rats of normal control group were subcutaneously injected with saline solution of the same dosage. The rats of high- and low-dose treatment group were respectively administered with gastric perfusion of high- and low-dose Yangxueqingnao granules once 10 min after the modeling. The rats of high- and low- dose prevention group were respectively administered with gastric perfusion of high- and low- dose Yangxueqingnao granules for 7 d before the modeling. The rats were sacrificed and the brain tissues were collected at corresponding time points. Related behavior indexes of rats in each group were observed and recorded. TNC c-Fos protein expression changes were measured and compared among different groups.  Results  At all time points, the c-Fos expression level of model group had no statistical difference from high- and low-dose treatment group (P > 0.05, for all). However, it was higher than that in high-dose prevention group (P = 0.031, 0.000, 0.000), while was only lower than that in low-dose prevention group at 60 and 180 min after modeling (P = 0.000, for all). There was statistical difference between high- and low-dose prevention group only at 30 min after modeling (P = 0.029). Conclusions  The Yangxueqingnao granules can significantly decrease TNC c-Fos protein expression, which suggests that the preventive effect of Yangxueqingnao granules may be greater than the therapeutical effect on migraine. Its preventive effect may be associated with the decrease of TNC c-Fos protein expression.

Key words: Migraine, Trigeminal nuclei, Proto-oncogene proteins c-fos, Disease models, animal

摘要： 目的 观察养血清脑颗粒对硝酸甘油偏头痛模型大鼠脑干三叉神经脊束核c-Fos 蛋白表达变化的影响。方法 共90 只雄性Wistar 大鼠，分别于皮下注射硝酸甘油建立偏头痛动物模型之前（预防组）、模型制备后（治疗组）接受高剂量（0.32 g/ml）或低剂量（0.16 g/ml）养血清脑颗粒治疗，行为学评价后行免疫组织化学染色检测大鼠三叉神经脊束核神经元c-Fos 蛋白表达水平。结果 模型组大鼠各观察时间点（30、60、180 min）三叉神经脊束核神经元c-Fos 蛋白表达水平与高、低剂量治疗组之间差异无统计学意义（均P > 0.05）；但高于高剂量预防组（P = 0.031，0.000，0.000），并于制模后60 和180 min时高于低剂量预防组（均P = 0.000）；制模后30 min 时高剂量预防组大鼠三叉神经脊束核神经元c-Fos 蛋白表达水平低于低剂量预防组（P = 0.029）。结论 预防性应用养血清脑颗粒可以显著降低三叉神经脊束核c-Fos蛋白表达水平。提示养血清脑颗粒对偏头痛的预防作用大于治疗作用，其机制可能与降低三叉神经脊束核c-Fos蛋白表达水平有关。

关键词: 偏头痛, 三叉神经核, 原癌基因蛋白质c-fos, 疾病模型, 动物