Abstract:
Objective To study the sensitivity of primary human glioma cell to chemotherapeutic agents in vitro in order to provide an experimental basis for clinical chemotherapy of glioma. Methods The glioma cells derived from 46 cases of fresh brain glioma specimens were cultured in vitro. The sensitivity of primary cultured brain glioma cell to 4 chemotherapeutic agents which were teniposide (VM26), nimustine (ACNU), methotrexate (MTX) and temozolomide (TMZ) was determined by flow cytometry assay. Results The sensitivity sequence in 46 cases of 4 chemotherapeutic agents from high to low was VM26 (39.13%), TMZ (34.78%), ACNU (26.09%) and MTX (6.52%), there was no significant difference in the chemosensitivity in the groups of VM26, ACNU and TMZ. The chemosensitivity in MTX group was significant lower than the other groups ( χ2 = 13.883, 11.210, 6.452; P < 0.05, for all). There was no significant influence of patients' sex and age on chemosensitivity (P > 0.05, for all). Except TMZ group, the other chemotherapeutic agents showed no significant correlation of chemosensitivity with the pathological grade of the tumor. Conclusion Glioma sensitivity to chemotherapeutic drugs in vitro is different. VM26 anti?glioma effect is stronger than the other 3 drugs; TMZ shows a better effect in high grade gliomas. Flow cytometry assay is a rapid drug sensitivity detecting method for screening chemotherapy drugs.
Key words:
Glioma,
Antineoplastic combined chemotherapy protocols,
Drug screening assays, antitumor,
Flow cytometry
摘要： 目的 观察不同抗肿瘤药物在体外对原代人胶质瘤细胞的敏感性，为临床筛选化疗药物、制订个体化方案提供指导。方法 46 例经手术后病理证实的胶质瘤新鲜组织标本进行体外原代培养，流式细胞术Annexin V-FITC/PI 双染法检测替尼泊苷、尼莫司汀、甲氨蝶呤和替莫唑胺在体外对原代胶质瘤细胞的敏感性；分析不同化疗药物敏感性与患者性别、年龄和肿瘤病理分级之间的关系。结果 人胶质瘤细胞对4 种化疗药物敏感性的强弱程度依次为替尼泊苷（39.13%）、替莫唑胺（34.78%）、尼莫司汀（26.09%）和甲氨蝶呤（6.52%），组间差异具有统计学意义（χ2 = 14.770，P = 0.002）；甲氨蝶呤组患者对化疗药物敏感性最低，与其他各组比较差异有统计学意义（χ2 = 13.883，11.210，6.452；均P < 0.05）。亚组分析提示，性别和年龄对化疗药物敏感性无显著影响（均P > 0.05），但不同WHO 病理分级对替莫唑胺化疗敏感性存在差异并具有统计学意义（χ2 = 4.305，P = 0.038）。结论 不同胶质瘤个体对化疗药物的敏感性不同，以替尼泊苷抗胶质瘤作用较强，替莫唑胺对高级别胶质瘤药物化疗效果更佳。流式细胞术可作为快速化疗药物敏感性检测方法，为胶质瘤筛选化疗药物并制订个体化方案。
关键词:
神经胶质瘤,
抗肿瘤联合化疗方案,
药物筛选试验,
抗肿瘤,
流式细胞术
WANG Ning, WANG Wei, YANG Ri-xiao, WANG Mao-de, WANG Tuo, CHEN Wei, LI Qi. Study on the sensitivity of primary glioma cell to chemotherapeutic agents in vitro[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2010, 10(5): 540-543.
王宁，王伟，杨日晓，王茂德，王拓，陈伟，李奇. 原代胶质瘤细胞化疗药物敏感性研究[J]. 中国现代神经疾病杂志, 2010, 10(5): 540-543.