Abstract: This study aims to explore the changes and clinical significances of T helper 17 (Th17) cells in the peripheral blood of patients with acute cerebral hemorrhage. The percentage of Th17 cells were assessed by flow cytometry. The mRNA levels of retinoid-related orphan receptor γt (RORγt) were detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The serum levels of interleukin-17 (IL-17) were measured by enzyme-linked immunosorbent assay (ELISA). According to the examination results, the percentage of Th17 cells, the mRNA expression of RORγt and the serum levels of IL-17 at 24 h, 3 d and 7 d increased significantly in patients with acute cerebral hemorrhage than in normal controls (P = 0.000, for all). Although the patients with acute cerebral hemorrhage had no significant differences in the percentage of Th17 cells, the mRNA expression of ROR γ t comparable with normal controls (P = 0.058, 0.239), the serum levels of IL-17 in patients were statistically higher than those in normal controls at 14 d (P = 0.000). The percentage of Th17 cells and the serum levels of IL-17 increase in the peripheral blood of patients with acute cerebral hemorrhage, suggesting Th17 cells may participate in the occurrence and development of cerebral hemorrhage.

Key words: Cerebral hemorrhage, T-lymphocytes, helper-inducer, Interleukin-17, RNA, messenger

摘要： 探讨脑出血急性期外周血辅助T 细胞17（Th17）表达变化及其临床意义。分别采用流式细胞术、实时定量逆转录-聚合酶链反应和酶联免疫吸附试验检测急性脑出血患者外周血Th17 细胞比例及核转录因子维A 酸相关孤儿受体γt（RORγt）mRNA 和IL-17 表达水平。结果显示，脑出血后Th17 细胞比例及RORγt mRNA 和IL-17 表达水平持续升高，并于脑出血后24 h 及第3 和7 天显著高于正常值范围（均P = 0.000）；至脑出血后第14 天时Th17 细胞比例和RORγt mRNA 表达水平降至正常水平（均P > 0.05），而外周血IL-17 表达水平仍高于正常值范围且差异有统计学意义（P = 0.000）。提示脑出血急性期外周血Th17细胞比例及IL-17表达水平升高可能参与了急性脑出血的病理进程。

关键词: 脑出血, T 淋巴细胞, 辅助诱导, 白细胞介素17, RNA, 信使