Abstract: Background Nowadays, the early diagnosis of tuberculous meningitis (TBM) is still one of the clinical difficulties, so the research focus is to seek out a specific marker of early TBM. This study is to evaluate the value of culture filtrate protein -10 (CFP-10) and Ag85 complex in cerebrospinal fluid (CSF) for the diagnosis of TBM. Methods According to the diagnostic criteria of TBM, 86 patients were divided into 3 groups: 30 patients with TBM; 27 with intracranial infection of non-TBM; 29 controls. CSF samples were collected by standard lumbar puncture and were used for detection of CFP-10 and Ag85 by enzyme-linked immunosorbent assay (ELISA). Results The median concentrations of CFP-10 and Ag85 in CSF of the TBM group were 0.74 and 1.10 pg/ml. The average concentrations of CFP-10 and Ag85 in the intracranial infection of non-TBM group and control group were (0.02 ± 0.01), (0.54 ± 0.10) and (0.02 ± 0.01), (0.52± 0.11)pg/ml respectively. The levels of CFP-10 and Ag85 in the TBM group were higher than that in the intracranial infection of non-TBM and the control group, and the differences were statistically significant (H = 60.958, P = 0.000; H = 57.972, P = 0.000), but there was no significant difference bewteen the intracranial infection of non-TBM group and the control group (t = 1.128, P = 0.253; t = 0.980, P = 0.329). Conclusion The levels of CFP-10 and Ag85 in the TBM group were significantly different from non-TBM group and control group. Detection of the two markers can be helpful in the early diagnosis of TBM.

Key words: Tuberculosis, meningeal, Cerebrospinal fluid, Bacterial proteins, Enzyme-linked immunosorbent assay

摘要： 研究背景 结核性脑膜炎的早期诊断目前仍是临床难点，寻找结核性脑膜炎早期特异性诊断指标是目前研究热点。本文主要探讨脑脊液培养分泌蛋白-10（CFP-10）和Ag85蛋白复合物表达水平对结核性脑膜炎的临床诊断价值。方法 采用酶联免疫吸附试验分别检测结核性脑膜炎（30例）、非结核性颅内感染（27例）和对照（29例）受试者脑脊液CFP-10和Ag85蛋白复合物表达水平。结果 结核性脑膜炎组患者脑脊液 CFP-10和Ag85中位表达水平分别为0.74 和1.10 pg/ml，均高于非结核性颅内感染组［（0.02 ± 0.01）、（0.54 ± 0.10）pg/ml］和对照组［（0.02 ± 0.01）、（0.52 ± 0.11）pg/ml］，组间差异有统计学意义（H = 60.958 ，P = 0.000；H = 57.972 ，P = 0.000）。非结核性颅内感染组与对照组之间差异无统计学意义（t = 1.128，P = 0.253；t = 0.980，P = 0.329）。结论 结核性脑膜炎患者脑脊液CFP-10和Ag85蛋白复合物表达水平高于非结核性颅内感染患者和对照者，检测这两项实验室指标的变化可协助结核性脑膜炎的早期诊断。

关键词: 结核, 脑膜, 脑脊髓液, 细菌蛋白质类, 酶联免疫吸附测定