摘要：

目的 观察未成熟癫痫大鼠血清神经元特异性烯醇化酶（NSE）、S-100B 蛋白（S-100B）、胶质纤维酸性蛋白（GFAP）表达变化，探讨其对未成熟脑癫发作的意义。方法 采用三氟乙醚诱导建立未成熟大鼠癫痫模型，按照随机数字表法随机分为对照组和惊厥组（单次惊厥组和反复惊厥组），酶联免疫吸附试验分别检测大鼠发作第1，2，7 和15 天时血清NSE、S-100B、GFAP 表达变化。结果 与对照组相比，单次惊厥组大鼠于惊厥发作第1 天血清NSE［（8.57 ± 0.56）μg/L］和S-100B［（0.45 ± 0.06）μg/L］表达水平即升高（均P = 0.000），此后逐渐下降至正常水平（均P > 0.05）；反复惊厥组大鼠于惊厥发作第1 天时血清NSE［（9.33 ± 0.61）μg/L］和S-100B［（0.78 ± 0.10）μg/L］表达水平即达峰值（均P = 0.000），此后逐渐下降，至第7 和15 天时降至正常水平（均P > 0.05），血清GFAP 表达水平自反复惊厥发作第1 天即升高［（0.44 ± 0.05）μg/L，P = 0.004］，至第7 天达峰值水平［（0.63 ± 0.08）μg/L，P = 0.000］，此后逐渐下降但至第15 天仍高于对照组［（0.40 ± 0.05）μg/L，P = 0.018］。结论 NSE 和S-100B 为单次惊厥发作性脑损伤的高敏感性生物学标志物，反复惊厥发作可使脑损伤程度加重，GFAP 表达变化不具有脑损伤预测作用。

关键词: 癫痫, 磷酸丙酮酸水合酶, S100 蛋白质类, 神经胶质原纤维酸性蛋白质, 疾病模型, 动物

Abstract:

Objective  To observe the dynamic changes of neuron-specific enolase (NSE), S-100B protein (S-100B) and glial fibrillary acidic protein (GFAP) in the serum of immature epileptic rat model, so as to explore the significance of these biochemical indexes on the damage of immature brain after epileptic seizures. Methods  The immature epileptic rat model was established by inducing flurothyl to 5-day-old specific pathogen free (SPF) Sprague-Dawley (SD) rats. The experimental animals were randomly divided into 2 groups: control group (N = 8) and seizure group (N = 64). Furthermore, the latter was subdivided into single seizure group (N = 32) and repeated seizures group (N = 32). Double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the changes of serum NSE, S-100B and GFAP in control group, single seizure group and repeated seizures group, on the 1st, 2nd, 7th and 15th days of the seizure onset.  Results  Compared with control group, the concentrations of serum NSE and S-100B in single seizure group increased significantly on the 1st day of onset (P = 0.000, for all), which respectively rose up to (8.57 ± 0.56) μg/L and (0.45 ± 0.06) μg/L, and then declined gradually to normal (P > 0.05, for all), while no obvious changes of GFAP was found. Compared with control group, the concentrations of serum NSE and S-100B in repeated seizures group reached the peak on the 1st day of onset [(9.33 ± 0.61) μg/L and (0.78 ± 0.10) μg/L; P = 0.000, for all], and then declined gradually to normal on the 7th and 15th days (P > 0.05, for all). Compared with control group, the concentration of serum GFAP in repeated seizures group increased significantly on the 1st day of onset [(0.44 ± 0.05) μg/L, P = 0.004], reached the peak on the 7th day [(0.63 ± 0.08) μ g/L, P = 0.000], then declined gradually, but was still significantly
higher than that in control group on the 15th day [(0.40 ± 0.05) μg/L, P = 0.018]. Conclusions  NSE and S-100B are highly sensitive biochemical markers of brain damage caused by single convulsive seizure. Repeated convulsive seizures could aggravate brain damage. GFAP is not a sensitive predictive indicator
on brain damage caused by single convulsive seizure.

Key words: