Table of Content

25 November 2008, Volume 28 Issue 11

Previous Issue    Next Issue

研究论文

Effect of human bone marrow mesenchymal stem cells on behavioral changes of cerebral ischemic rats

Jun-ji WEI; Li-fen ZENG; Ren-zhi WANG; Chun-hua ZHAO; Ming FENG; Yu WANG; Gui-lin LI; Wan-chen DOU; Yan-guo KOU

2008, 28(11):  1121-1124. 

Asbtract ( 1857 )  

Related Articles | Metrics

Objective Investigate the behaviors of the cerebral ischemic rats after treatment with bone marrow mesenchymal stem cells(BMSCs). Methods Bone marrow of volunteer was collected and BMSCs were separated and cultivated. 24 adult male Sprague-Dawley rats were performed transient (2 hours) middle cerebral arterial occlusion(MCAO) and divided into treated group (n=12) and control group (n=12) .All the rats received corresponding behavioral training before surgery. 15?lhBMSCs (2×10 10cells/L) and D-hanks(15?l) were injected into the brain cortex after 24h of MCAO. Morris water maze test, NSS, Rotarod test and adhesive-removal test were performed serially and cyclically from the 4th day after transplantation. Result From the 8th day after transplantation, the mean escape time and the mean swimming distance of treated group is shorter significantly than control's in Morris water maze test（p<0.05）. From the 10th day, the mean latency period of treated group is longer significantly than control's in rotarod test with 10r/min. From the 13th day, the mean latency period of treated group is shorter significantly than control's in adhensive-removal test. Only two time points is different in NSS between the two groups. Conclusion Transplantation of BMSCs can improve the ability of learning and memory as well as the sensorimotor function recovery after stroke.

MAR Increased Transgene Expression in Stable Transfected CHO Cells

Tian-yun WANG; Zhong-min HAN; Xian-jun YANG; Zhong-sheng DONG; Qing-yi WANG

2008, 28(11):  1125-1128. 

Asbtract ( 1765 )  

Related Articles | Metrics

Objective To study the effect of human β-globin matrix attachment region (MAR) on transgene expression in stably transfected CHO cells. Methods The eapression vector was constructed , which contained the β-globin MAR in both sides of Chloramphenicol acetyltransferase (CAT) reporter gene expression cassette in cis, then transfected into CHO cells and screened under G418 selection. The CAT reporter gene expression was analyzed through ELISA method. Results It has showed that theβ-globin MAR could enhance the CAT gene expression 5.5-fold in stably transformed CHO cells, while the transgene expression variation among individuals of transformants was decreased. Conclusion MAR could increase transgene expression in stably transfected CHO cells.

Rosiglitazone depressed activation of rat hepatic stellate cells in vitro

Yan-tong GUO; Jing-ming ZHAO; Lei SONG; Mai ZHOU; Gang-jun JIAO; Tao LI; Xi-sheng LENG

2008, 28(11):  1129-1133. 

Asbtract ( 2009 )  

Related Articles | Metrics

Objective To investigate the mechanism of effect of rosiglitazone (PPAR ligand) on rat hepatic stellate cells (HSCs) in vitro. Methods The primary cultured HSCs were randomly divided into three groups: the control group, TGF-β1（5?g/L）group, TGF-β1 with 10?mol/L rosiglitazone group. Detecting the samples 48 hours after the HSCs were treated with TGF-β1（5ng/ml）and TGF-β1 with 10?mol/L rosiglitazone respectively: The expression of type Ⅰ pro-collagen was detected by reverse-transcription polymerase chain reaction (RT-PCR). The expressions of SMAD3、SMAD4、SMAD7、α-smooth muscle actin (α-SMA) and type Ⅰcollagen were measured by using Western blot assays. The expression of α-SMA was marked by immunofluorescence and observed under confocal microscope. The cell proliferation was determined with MTT colorimetric assay. Results TGF-β1 significantly increased the proliferation of HSCs and thus promoted HSCs to express type Ⅰcollagen and α-SMA（P<0.01）; these effects of TGF-β1 were suppressed by rosiglitazone（P<0.01）. The difference of expression of SMAD3、SMAD4、SMAD7 in each group was not statistically significant. Conclusion PPAR ligand can suppress the activation of TGF-β1 on HSCs.

Impairment of lymphoproliferative response to human herpesvirus-6 in oral squamous cell carcinoma patients

Fang WANG; Kun YAO; Feng ZHOU; Jie YANG

2008, 28(11):  1134-1137. 

Asbtract ( 458 )  

Related Articles | Metrics

Objective To study the lymphoproliferative responses to HHV-6 （Human Herpesvirus-6, HHV-6）among patients with oral squamous cell carcinoma, and discuss the role of HHV-6 in the pathogenesis of oral squamous cell carcinoma. Methods IgG antibody to HHV-6 in plasma were identified by means of indirect immunofluorescence (IIF). Immuno-magnetic beads were used to prepare CD4+ T cells and CD8+ T cells. The proliferation of CD4+ T cells, CD8+ T cells and PBMCs stimulated with HHV-6 or anti-CD3 antibodies were measured by means of 3H-thymidine uptake. The percentage of CD4+ CD25+ Treg cells（Regulatory T cell,Treg）within the peripheral blood CD4+ T cell compartment was analyzed by flow cytometry. Results Significantly higher proportion of patients with oral squamous cell carcinoma had IgG antibody to HHV-6 (8/8 ) in plasma compared with those in control subjects (12/20 )；The proliferative responses of PBMCs and CD4+ T cells from patients withoral squamous cell carcinoma to HHV-6 were significantly decreased compared to those from HHV-6-infected and uninfected healthy individuals（P＜ 0.05；P＜ 0.05）. The proliferative responses of PBMCs and CD4+ T cells from HHV-6-infected healthy individuals to HHV-6 were significantly decreased compared to those from HHV-6-uninfected healthy individuals（P＜ 0.05）；Patients with oral squamous cell carcinoma had a significantly higher percentage of CD4+ CD25+ Treg cells within the peripheral blood CD4+ T cell compartment compared with HHV-6 infected and uninfected healthy individuals did. Conclusion These results demonstrated that impairment of HHV-6-specific CD4+ T cell immune responses in patients with oral squamous cell carcinoma, therefore, HHV-6 possibly plays a role in the pathogenesis of oral squamous cell carcinoma.

Apelin-13 Microinjection into the Paraventricular Nucleus Aggravated Gastric Mucosal Cellular Apoptosis Induced by Gastric Ischemia-Reperfusion in Rats

Lei DING; Jian-fu ZHANG; Yong-mei ZHANG

2008, 28(11):  1138-1141. 

Asbtract ( 443 )  

Related Articles | Metrics

Objective　To observe effects and mechanism of Apelin-13 microinjection into the hypothalamic paraventricular nucleus on gastric ischemia-reperfusion injury in rats. Methods 　 After Apelin-13 was microinjected into PVN, the experimental model of GI-R was established by clamping the celiac artery for 30 min and then reperfusing the artery for 1 h. We used immunohistochemistry to detect the gastric mucosal cells apoptosis, proliferation and the expression of BCL-2, BAX. Results (1) Apelin-13 microinjection into the PVN could aggravate GI-R injury in an dose-dependent manner by using 0.2 , 1.0 or 5.0 μg, respectively. (2) Compared with GI-R group, Apelin-13 microinjection into the PVN markedly increased gastric mucosal cellular apoptosis, decreased the proliferation, and promoted the protein expression of BAX, but obviously inhibited the protein expression of BCL-2. Conclusion Apelin-13 microinjection into the PVN could aggravate GI-R injury by promoting gastric mucosal cellular apoptosis and inhibiting proliferation.

Signaling pathway in human airway mucous hypersecretion

Xiao-ling WU; Xiang-dong ZHOU

2008, 28(11):  1142-1145. 

Asbtract ( 473 )  

Related Articles | Metrics

Objective To explore the upstream signal transduction pathway of neutrophil elastase（NE）-induced mucin(MUC)5AC gene expression. Methods The well cultured A549 cells were either incubated with NE alone or with the addition of DMTU, aprotinin or AG1478. The levels of MUC5AC mRNA were measured with RT-PCR. The activation of epidermal growth factor receptor(EGFR) and signaling were assessed by measuring the release of epidermal growth factor(EGF) and the phosphorylation of EGFR. Results NE increased MUC5AC gene expression accompanied by the increase of EGF and phosphorylated EGFR levels. DMTU, aprotinin and AG1478 significantly inhibited the increase of MUC5AC gene expression. DMTU and aprotinin significantly decreased the levels of EGF and phosphorylated EGFR. Conclusion NE can induce MUC5AC gene expression via EGFR signalling in A549 cells. The upper stream involves oxidants, activation of tissue kallikrein and EGF.

Bioinformatics Analysis of Human Glucocoiticoid Receptor Regulated Target Genes

Ling ZENG; Wei GU; Jun YAN; Hai-yan WANG; Jian-yun ZHOU; Jian-xin JIANG

2008, 28(11):  1146-1150. 

Asbtract ( 479 )  

Related Articles | Metrics

Objective To identify the distribution and function of glucocoiticoid receptor (GR) regulated target genes in the human genome, and to know the bioinformatic significance of it. Methods: With bioinformatics methods, we found out all the genes that contains Glucocorticoid Response Element's (GRE) consensus sequences, and extend our study to the distribution of consensus sequences in the human genome and the functions , protein products and the Gene Ontology (GO) number of the GR target genes and then to emphasize these genes' function in inflammation and immunity. Results: We found 225 genes which contain the GRE-1 consensus sequence (5'-AGAACAnnnTGTTCT-3') and 94 genes contain GRE-2 consensus sequence (5'-GGTACAnnnTGTTCT-3'). Conclusion: According to the analysis of the function of genes which contain the consensus sequence of GRE, F2R, DAXX, PAG1, ULBP3, OR8D4, RIMS1, NPY2R are correlate with inflammation and immunity.

FRNK inhibited Hepatic Stellate Cells in Vitro

Jiang-gang SHEN; Xiao-lan ZHANG; Xiao-xia HUO; Qing-hai JIAO; Hong-fang WANG

2008, 28(11):  1151-1155. 

Asbtract ( 373 )  

Related Articles | Metrics

Objective To investigate the effect and mechanism of FRNK on proliferation of Hepatic Stellate Cells in vitro. Methods After FN stimulated HSCs, FRNK plasmid mediated by cationic liposome was transfected into HSCs. The proliferation of HSCs was evaluated by improved MTT assay. And the levels of FRNK, FAK, p-FAK (Tyr397), ERK1 and p-ERK in HSCs were assayed by Western blot and RT-PCR. Results Compared with nFRNK plasmid group，FRNK inhibited the proliferation of HSCs，and the inhibition rates at 12，24 and 48 h were 20.07 %, 26.16 % and 29.77 %( P<0.01), respectively. Compared with the nFRNK plasmid group, the expression of p-FAK, ERK1 and p-ERK in FRNK plasmid group reduced; On the contrary, compared with the control group, the expression of p-FAK, ERK1 and p-ERK in FN group increased. Conclusions FRNK can inhibit the HSCs proliferation in a time-dependent manner and FAK-ERK signal transduction pathway was perhaps involved in the process.

Effect of PARP inhibitor 5-AIQ on PARP/NF-κB complex and NF-κB activity in murine colon carcinomaCT26 cells

Li CAI; Ya-lan WANG; Xiao LIN

2008, 28(11):  1156-1159. 

Asbtract ( 357 )  

Related Articles | Metrics

Objective: The aim is to investigate the effect of PARP inhibitor 5-AIQ on PARP/NF-κBp65 complex and the NF-κB activity in murine colon carcinoma CT26 cell lines．Methods: The murine colon carcinoma CT26 cell lines were treated with 5-AIQ in vitro．The expressions of PARP and NF-κBp65 were analyzed by Western Blot. The PARP/NF-κBp65 complex was analyzed by co-immunoprecipitation. The NF-κB binding activity was detected by electrophoretic mobility shift assay (EMSA). Results: Compared with 5-AIQ-untreated group, the expressions of PARP and NF-κBp65 were markedly decreased in 5-AIQ-treated colon carcinoma CT26 cell groups (P<0.05). The PARP/NF-κBp65 complex was decreased in 5-AIQ-treated colon carcinoma CT26 cell group (P<0.05).The NF-κB DNA binding activity was also reduced in 5-AIQ-treated colon carcinoma CT26 cell groups(P<0.05). Conclusions：These results suggest that 5-AIQ can inhibit PARP expression in murine colon carcinoma CT26 cells, and further inhibit the formation of PARP/NF-κBp65 complex and NF-κB activity.

IL-10 reduce Interleukin-1β in rats with acute necrotizing pancreatitis

De-hai DENG; Zhi-hai LIANG; Guo-du TANG

2008, 28(11):  1160-1163. 

Asbtract ( 351 )  

Related Articles | Metrics

Obsjective To investigate the effects of recombinant human interleukin(IL)-10 on serum Interleukin-1β in L-arginine-induced acute necrotizing pancreatitis in rats. Methods Ninty-two Sprague-Dawley rats were randomly divided into three groups. Group A(n=36) and Group I(n=32) received three intraperitoneal injections of 6% L-arginine (1.0mg/g) at hourly intervals. Group I(n=32) was treated with 10,000 unites of intraperitioneal recombinant human IL-10 at the 2nd, 5th and 8th hour after the last injection of L-arginine. Group C(n=24) received saline alone. Rats were killed at the 4th, 12th, 24th and 36th hour after the last L-arginine injection. Serum interleukin-1β and amylase were assayed.Pancreatic tissues were fixed in formalin. Histopathological score were recorded according to the edema, inflammation and necrosis of the tissues in three groups. Results Compare with group C, serum amylase and interleukin-1β and pancreatic histopathological scores in group A increased significantly after L-arginine injection(P<0.05 or P<0.01). Compared with group A, the levels of serum amylase and interleukin-1β in group I were significantly decreased at various time points (P<0.05 or P<0.01). Pancreatic histopathological scores in group I were decreased compare with group A at the 12th, 24th, 36th hour(P<0.05 or P<0.01). Conclusions IL-10 attenuates the severity of experimental acute necrotizing pancreatitis by inhibiting the release of interleukin-1β.

Changes of Th1/Th2 in peripheral blood in early stage of allogeneic orthotopic rat liver transplantation

Zhen-lin YANG; Guang-he CUI; Zhong-ming JIA; Xi-long WANG; Yong HAN

2008, 28(11):  1164-1168. 

Asbtract ( 404 )  

Related Articles | Metrics

Objective To investigate whether the tendency of Th1/Th2 cell in peripheral blood associatied with different immune states in early stage of allogeneic orthotopic rat liver transplantation . Methods Allografted rats were divided to three groups(each group n=36): syngeneic control(group A), the group of allograft treated with CsA 2mg kg-1.d-1(group B) and allograft (group C). At post-transplantation day of 1,3,5,7,14, six rats of each group were respectively sacrificed to harvest allografted liver for pathological , percentage of CD4+CD45RC± T cell subsets in peripheral blood of recipient by flow cytometry , and additional subgroups (n=6) for observation of survival time. Results (1)The survival time of both group A and group B were significantly longer than that of group C (>100day vs 34.3 3.6days, p<0.01).(2)Apart from 1 day, the rejection grades were higher in the group C and B than that in group A and there were significant differences between the two groups（p<0.05 or<0.01）. (3)From third day to fourteenth day post-transplantation, the ratio of CD4+CD45RC+ percentage with CD4+CD45RC- percentage was significant higher in group C than that in group B and group A（p<0.05 or 0.01）.（4）There was negative correlation between ratio of CD4+CD45RC+%/CD4+CD45RC-% and rejection grades（r=-0.565，p<0.01）, but positive correlation existed between ratio of CD4+CD45RC+%/CD4+CD45RC-% and rejection grades（r=0.745,p<0.01）。Conclusions The ratio of Th1/Th2 cell in peripheral blood has different changes according to different immune states in early stage of allogeneic orthotopic rat liver transplantation .

Morphology，Ultrastructure and Apoptosis of Freeze-dried and Rehydrated Platelets

Ying LIU; Xian-guo XU; Xiao-zhen HONG; Kai-rong MA; Fa-ming ZHU; Li-xing YAN

2008, 28(11):  1169-1173. 

Asbtract ( 475 )  

Related Articles | Metrics

Objective To evaluate the effect of lyophilization on the morphological feature, ultrastructure and apoptosis of platelets. Methods The lyophilized platelets were rehydrated after preserved 30 days. The changes of the morphology and ultrastructure of platelets were observed under the light microscope and transmission electron microscope (TEM) respectively. The survival rate and apoptosis rate of platelets were analyzed by flow cytometry (FCM). The aggregation rates of rehydrated lyophilized platelets were measured by using 1U/ml thrombin. Results The morphology of rehydrated lyophilized platelets keeps normal. There were no evident cell aggregation in rehydrated lyophilized platelets，but the refractive index of them was relative weak compared with fresh platelets. The result of ultrastructure observation indicated that the intracellular elementary structures of rehydrated lyophilized platelets were clear and the cell membrane keeps intact. According to the result of FCM analysis, the apoptosis rate of the fresh platelets and lyophilized platelets was(0.44±0.19)％ and (3.25±1.68)％ respectively, and there was a significant difference between the two groups（P＜0.05).The maximum aggregation rates of rehydrated lyophilized platelets were(93.28±2.3)％. Conclusion The rehydrated lyophilized platelets keep intact cellular structure. The study provides an evidence for successful building the method of lyophilized platelet in our lab.

Therapeutic application of plasmid DNA coding for human Fas ligand in animal model for thyroid-associated ophthalmopathy

Xiu-juan LI; Chun LIU; Hui ZHANG; Hua SUI; Sheng-hua ZHAN

2008, 28(11):  1174-1177. 

Asbtract ( 394 )  

Related Articles | Metrics

Objective To investigate therapeutic application of eukaryotic expression vector pcDNA3.1(+)/hFasL in animal model for TAO. Methods Three groups were set in this study. Animals in control group (10 mice) were immunized with splenocytes sensitized by blank plasmid pcDNA3.1(+), and then treated with pcDNA3.1(+). Animals in treated group (19 mice) and model group (19 mice) were all immunized with splenocytes sensitized by hTSHR, then the former were injected behind the eyeballs with pcDNA3.1(+)/hFasL, while the latter had no special treatment. Results 52.6％ in model group displayed obvious edema, hyperplasia of adipose tissue, focal degeneration and disruption of muscular fibers in their orbital tissues, and when compared with the control group, elevated TT4 and reduced TSH levels (p<0.05) were also observed. In treated group, only 15.8％ showed changes of TAO with apoptosis was found, and there was no statistical significance in levels of TT4 and TSH between treated group and control group. TRAb levels were not statistically significant among the three groups. Conclusion Retrobulbar injection with the plasmid coding for hFasL has made a curative effect on TAO in mice. These results indicated the therapeutic potential of gene therapy via Fas/FasL-mediated pathway for TAO.

Cobra venom factor inhibited inflammatory responses in septic rats

Rui-lan WANG; Qiao WEI; Fu-xin KANG; Shu CAO; Guo-ping LI; Song-wei RU; Cai-hua LI

2008, 28(11):  1178-1182. 

Asbtract ( 471 )  

Related Articles | Metrics

Objective　To investigate the effects on release of blood-serum cytokines and lung tissues by inhibition of complement activation with cobra venom factor(CVF)in septic rats. Methods　One hundred and forty-four SD rats(clean, male,250-300g) were randomly divided into three groups(each n=48):group A(sham group); group B(sepsis group):sepsis was induced by cecal ligation and puncture (CLP) ; group C(CVF pretreatment):sepsis was induced by CLP after pretreatment with CVF，rats were given a dose of 50μg/kg CVF before operation, an equal volume of saline solution in group A and group B. MPO contents, levels of TNF-α、IL-6 and IL-10 were measured at 6 hours points. Lung tissues were observed the changes of histopathology. Results The manifestations of sepsis were severe in group B than that of group A. The levels of TNF-αand IL-6 were higher in group B than that of group A（P< 0.05 ，P< 0.01）. MPO contents of lung tissues were higher in group B than that of group A（P< 0.05 ,P< 0.01）.The degree of histological damage were much severe in group B than in group A. However, after injection CVF in group C, the manifestations of sepsis were much lighter, the levels of TNF-a、IL-6 and MPO were decreased obviously (P< 0.05,P< 0.01), and the histologic damage ameliorated in CVF group compared to that of group B. Conclusion CVF could lessen the systemic inflammatory response and provide pulmonary protection in septic rats.

Down regulation on endogenous apelin and its receptor expression in the rats with septic shock

Lin XUE; Chun-shui PAN; Xu TENG; Chun-yu ZHAO; Yan CAI; Chao-shu TANG; Yong-fen QIN

2008, 28(11):  1183-1186. 

Asbtract ( 377 )  

Related Articles | Metrics

Objective To observe the variation of endogenous Apelin with its APJ receptor on the model of rats with septic shock are caused by cecal ligation and puncture (CLP). Methods: Twenty male Wistar rats weighting 200~250g randomized into two groups; control group, severe septic shock group. Enzyme immunoassay (EIA) was used to detect levels of Apelin in plasma and myocardia. RT-PCR was used to analysis the relative amount of mRNA of Apelin and APJ in the myocardia. Western blot was used to determine the protein levels of myocardial APJ. Result: Compared with sham, rats with sepsis showed inhibited cardiac function (+LVdP/dtmax and -LVdP/dtmax decreased, and LVEDP elevated). Rats with septic shock showed significant hypoglycemia and hyperlacticaemia, lower levels of Apelin in plasma and myocardia, decreased mRNA relative amount of Apelin and APJ in the myocardia, and lower myocardial APJ protein level. Conclusion: Apelin and its receptor might play an important role in septic shock.

Differentiation of rat bone marrow mesenchymal stem cells to mesangial-like cells

Hong JING; Min WEI; Hong-mei SONG; Qiang QU

2008, 28(11):  1187-1191. 

Asbtract ( 415 )  

Related Articles | Metrics

Objective Bone marrow mesenchymal stem cells (BMSCs) have the potential to differentiate to glomerular mesangial cells. Based on established research, our study was aimed to reconfirm the mesangial cell differentiation potential of BMSCs, and study the mechanism of cell induction. Methods Rat BMSCs were isolated and cultured in the presence of platelet-derived growth factor (PDGF) -BB to induce the mesangial cell phenotype. On culture day 7, the cell morphology, mesangial cell-specific markers, and cell contraction function were examined. MAPK/ERK kinase inhibitor (PD98059) was added to examine its impact on cell growth and mesangial cells marker expression. Results After cultivation under the differentiation condition for 7 days, the induced cells expressed α-Actin and Desmin, which highly resembled cultured mesangial cells in rat. The induced cells with increasing level of PDGF receptors mRNA expression changed into a wide range of shapes from spindle to stellate and were contracted in response to angiotensin Ⅱ. PD98059 perturbed the induced cells mesangial cell-specific markers mRNA expression. Conclusion These data indicated that BMSCs can differentiate into mesangial-like cells with PDGF-BB induction in vitro. The mechanism involved in the differentiation was mediated through MAPK/ERK1/2 signal transduction pathway.

技术与方法

Prokaryotic Expression of Human nervous system polycomb 1(NSPc1) and Preparation of Anti-NSPc1 polyclonal Antibody

Yan-hua GONG; Xu WANG; Xu-dong WU; Bo-qin QIANG; Xiao-zhong PENG; Jian-gang YUAN

2008, 28(11):  1192-1196. 

Asbtract ( 393 )  

Related Articles | Metrics

Objective To express the human recombinant NSPc1 protein and prepare specific polyclonal antibody against it. Methods The recombinant expression plasmid pET43.1a-HIS-NSPc1 was made and transformed into E.coli.(BL21), and then the recombinant fusion protein HIS-NSPc1 was expressed and purified. Four New Zealand rabbits were immuniged with purified recombinant HIS-NSPc1 protein as antigen and polyclonal antibodies against NSPc1 were prepared. The specificity of the anti-sera were analyzed by Western Blot. Results The purity of the recombinant NSPc1 protein is up to about 95%. Rabbit against NSPc1 antibodies were obtained. Western blot showed that the antibodies were able to detect both endogenous and/or exogenous NSPc1. Conclusion The NSPc1 polyclonal antibodies prepared by using recombinant NSPc1 protein as antigen has better specificity to NSPc1.It can be used for functional analysis of NSPc1 in vivo and in vitro.

Culture and Identification of smooth muscle progenitor cells from rat marrow

Xue-feng BU; Yu-lan YAN; Yang LIU; Zhi-jiang ZHANG; Mu-bin WANG

2008, 28(11):  1197-1202. 

Asbtract ( 378 )  

Related Articles | Metrics

AIM To isolate and identify smooth muscle progenitor cells ( SPCs) from rat bone marrow and observe specific expression profile of the smooth muscle progenitor cells during proliferation and differentiation in vitro. Methods MNCs were isolated by ficoll density gradient centrifugation from rat marrow and cultured in conditioned nutrient chemical, identification was performed by immunofluorescent staining (α-SMA,CD14).And smooth muscle cells specific markers (α-SMA) were determined with Western blotting and Real-time PCR at different time. Results During culturing, cells adhered and became spindle shaped with outgrowth at 4 d and 7 d ,and presented typical "peak" "valley" at 14 d (3 generation). Both α-SMA and CD14 were positive after 4 d. Expression for α-SMA was not found at 1 d with Western blotting,but it gradually enhanced at 4 d and reached to the top from 10d to 14 d ,and still remained high-level at 21 d .The results with Real-time PCR indicated that the expression of α-SMA mRNA within non-induced cells was not found ,but after being induced it gradually enhanced at 4 d and got to peak at 14 d, and still remained high-level at 21 d , low-expression at 1 d was significantly different from the other ones (P<0.01). Conclusion SPCs could be isolated and cultured from rat marrow monocytes, and proliferated, differentiated into smooth muscle -like cells.

研究短文

Effect of Repetitive Limb Ischemia on Blood Pressure, Heart Rate, and Tissue Oxygen Index in Healthy Human

Sha DANG; Yu-min LUO; Xun-ming JI; Guo-wei LV; You-qin LIU; Wei-zhen NIU; Hai-shu DING

2008, 28(11):  1203-1204. 

Asbtract ( 409 )  

Related Articles | Metrics

Expression of CD147，MMP-2 and TIMP-2 in cutaneous

Hao-jing CHEN; Hai-long WU

2008, 28(11):  1205-1206. 

Asbtract ( 345 )  

Related Articles | Metrics

Cardioprotection role of calcium-activated potassium channel of mitochondria in remote ischemic-reperfusion in rat

Shi-zhong ZHANG; Yang CAO; Qiang XIA

2008, 28(11):  1207-1208. 

Asbtract ( 370 )  

Related Articles | Metrics

短篇综述

Regulation of TFPI-2 gene expression and its role in diseases

Qin ZHANG; Hong JIN

2008, 28(11):  1209-1211. 

Asbtract ( 498 )  

Related Articles | Metrics

Tissue factor pathway inhibitor-2 (TFPI-2) is a matrix-associated Kunitz-type serine proteinase inhibitor, which is considered to play an important role in such pathophysiological processes, including artherosclerosis, tumor metastasis and angiogenesis. Extracellular signals can regulate TFPI-2 gene expression through modulating promoter or signaling pathway or other factors. The mechanism, more over, has become one of the focus in recent years.

Research progresses on pyruvate dehydrogenase kinases

Ji-yong JIAN; Yong ZHANG; Da-hai ZHU

2008, 28(11):  1212-1215. 

Asbtract ( 655 )  

Related Articles | Metrics

Pyruvate dehydrogenase kinases (PDKs 1-4) can regulate the activity of the mitochondrial pyruvate dehydrogenase complex (PDC) to catalyse the oxidative decarboxylation of pyruvate, and then links glycolysis to the tricarboxylic acid cycle and ATP production. In this review, we summarize recent significant advances in our knowledge of the mechanisms regulating PDKs and the function of PDKs inhibitors in lowering blood glucose, decreasing damage during heart ischemia and also triggering apoptosis in cancer cells. PDKs will be a possible pharmacological targets in diabetes，heart ischemia and cancer therapy.

BKCa and Atherogenesis

Xian-gang MO; Xing-lin LUO

2008, 28(11):  1216-1218. 

Asbtract ( 444 )  

Related Articles | Metrics

Atherosclerosis is a kind of complex progressive inflammation. The exposure to atherogenic risk factors, particularly OxLDL , induces the activity of BKCa in endothelial cell, monocyte/macrophage(MФ) , vascular smooth muscle cell, platelet and orher cells to activate, which precipitates dysfunction of the cells and therefore contributes to the development of atherosclerosis. This article briefly reviews the reseach progress in BKca participating in the development of atherosclerosis.

Progress in the effects of CCN1 on cardiovascular system

Yang YU; Lan HUANG

2008, 28(11):  1219-1222. 

Asbtract ( 411 )  

Related Articles | Metrics

CCN1 is a novel extracellular matrix-associated signaling protein which possesses 381 amino-acid residues to compose 4 distinct structural modules with 38 conserved cysteine residues.This protein has a variety of properties in cardiovascular system, affecting the cellular behaviors such as differentiation, proliferation and migration of vascular endothelial cells, smooth muscle cells and cardiac myocytes, suggesting an essential roles of CCN1 in angiogensis, vascular injury, cardiac development and myocardial infarction.

Signal transduction passways of GAG in lysosome which inhibits apoptosis induced by oxidative stress

Gan-lin ZHANG; Xiao-li YUE; Ping LI

2008, 28(11):  1223-1225. 

Asbtract ( 392 )  

Related Articles | Metrics

Glycosaminoglycan(GAG) has recently become an important landscape for discovering new mechanisms of apoptosis stressed by oxidative stress. In this review, we focused the signal transduction passways of GAG in lysosome which can inhibit apoptosis induced by oxidative stress.