Table of Content

05 March 2010, Volume 30 Issue 3

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研究论文

Identification of nPKC -interacted proteins in the cortex of hypoxic preconditioned mice

Su-juan FENG; Xu LIU; Cai-yan ZHANG; Xiang-ning BU; Nan ZHANG; Yuan SUN; Fei GUO; Jun-fa LI

2010, 30(3):  225-231. 

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Objective Identify novel protein kinase C (nPKC )-interacted proteins in the cortex of hypoxic preconditioned mice. Methods The techniques of immunoprecipitation (IP) and two-dimensional electrophoresis (2-DE) combining with ImageMaster 2D Platinum software were applied to analyze the differential expressions of nPKC -interacted proteins; the target protein spots were identified by using matrix-associated laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and Western blot. Results Compared with control group, there were 34 upregulated protein spots and 20 downregulated protein spots in cytosolic fraction, while 27 upregulated prtein spots and 28 downregulated protein spots were determined in particulate fraction of cerebral cortex of HPC mice. The levels of nPKC -interacted HSP70 and 14-3-3 protein expressions increased significantly both in cytosolic and particulate fractions; but the protein level of nPKC -interacted HSP60 increased only in particulate fraction of cerebral cortex of HPC mice. Conclusion nPKC might be involved in the development of cerebral HPC via the regulation of its interacted proteins such as HSP60, HSP70 and 14-3-3 .

Analysis of Differential Urinary Proteome in Experimental Minimal Change Nephropathy

Yan WANG; You-he GAO

2010, 30(3):  232-236. 

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Objective To screen early urine protein markers for minimal change nephropathy. Method Adriamycin nephropathy was employed as minimal change nephropathy model. Urinary protein and ConA captured glycoproteins were respectively profiled. Result By profiling urine proteome, 25 differential proteins were identified. These differential proteins were from leaked plasma proteins, secreted proteins from immuno- and in?ammatory cells, specific secreted proteins from urinary tract, and et al. They take part in different pathogenic process, eg. hemodynamic changes, podocytes injury, immunological disorder and et al. By profiling ConA-enriched urinary glycoproteome, 21 differential proteins were identified, among which 12（57%）were different from the above 25 differential proteins. This indicates that the knowledge of urine glycoproteome is complementary to urine proteome in understanding kidney condition. Conclusion These differential proteins can be potential indicators of minimal change nephropathy, and can help better understand the pathogenesis by further studying their functions.

Lentivirus PARG-shRNA transfection decreases colon carcinoma lovo cells matrix adhesion, migration and invasion potencies

Qiao-zhuan LI; Ya-lan WANG; Xian LI

2010, 30(3):  237-241. 

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Objective To approach the effects of Poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion, migration and invasion. Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected. Western blot was used to test the expression of PARG, Poly(ADP-ribose) polymerase(PARP) and NF-κB. The lovo cell matrix adhesion, migration and invasion potencies were observed by cell matrix adhesion, migration and invasion assay. Results We obtained lovo cell strain with PARG gene silencing perpetually. The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (p<0.05, p<0.05). PARG gene silencing of lovo cells decreased the cell matrix adhesion, migration and invasion potencies. The inhibitory rates of lovo cell matrix adhesion, migration and invasion were 25.22%、38.71%、35.29% separately. Conclusion The data suggested that PARG gene silencing of lovo cells could inhibite the cell matrix adhesion, migration and invasion potencies, it probably related that PARG gene silencing decreased the activity of PARP and NF-κB. PARG probably plays an important role in the infiltration and metastasis of tumour.

Selecting the tag SNPs of TLR2 gene with bioinformatics techniques

Ke-hong CHEN; Ling ZENG; Wei GU; Shu-na HUANG; Qing LIU; Jian-xin JIANG

2010, 30(3):  242-245. 

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Objective To select the tag SNPs of TLR2 gene in the Chinese population with bioinformatics techniques. Methods We ascertained the assayed scope of the TLR2 gene with the aid of NCBI database and downloaded SNP genotype data of the TLR2 gene in the Chinese population from Hapmap database. Then a software package named Haploview (version 4.0) were used to calculate linkage disequilibrium (LD) statistics. Haplotype blocks were constructed throughout the TLR2 gene according to the upper and lower 95% confidence bound of the D' value. Meanwhile, we selected the tag SNPs based on r2 values and the LOD value between SNPs and picked up the representative haplotypes in accordance with the proportion of each haplotype in the haplotype blocks, respectively. Results We constructed 2 haplotype blocks within the TLR2 gene and selected 3 tag SNPs containing 3013 A/G, 19216 T/C, 22215 G/T in the Chinese population. Meanwhile, we identified the representative haplotypes of which the tag SNP would be on behalf of every haplotype block. Conclusion The SNPs of 3013 A/G, 19216 T/C and 22215 G/T, the most representative SNPs in the whole TLR2 gene in the Chinese population, could be selected as tag SNPs to guide their association studies between the TLR2 gene and sepsis.

Heme oxygenase-1 downregulates mucous hypersecretion induced by cigarette smoke in A549 cells

Rui-xia LEI; Xiang-dong ZHOU

2010, 30(3):  246-251. 

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Objective To investigate the effects of heme oxygenase-1(HO-1) on the signal transduction pathway of mucin(MUC) expression induced by cigarette smoke. Methods The cell model of mucous hypersecretion was made by human lung A549 cell stimulated by cigarette smoke extract(CSE), the cells were divided into 4 groups: a negative control group, a CSE treatment group, an hemin pre-treatment group and a ZnPPIX pre-treatment group. The expression of MUC5AC, epidermal growth factor receptor(EGFR), p-EGFR, HO-1 and dual oxidase 1(Duox1) were detected. The cell activity was assessed by methyl thiazolyl tetrazolium method. The changes of HO-1 mRNA, Duox1 mRNA, EGFR mRNA and MUC5AC mRNA were examined by the use of reverse transcriptase-polymerase chain reaction. The protein expression changes of EGFR, p-EGFR, Duox1 and HO-1 were measured by Western blot, while the protein expression changes of MUC5AC were detected by enzyme-linked immunosorbent assay. Results The expression levels of MUC5AC mRNA and its protein in the CSE group were 0.660+0.044 and 157+3 ?g/mg, both increased significantly (P<0.05) as compared to those in the control group[0.412+0.043, (105+8) ?g/mg]. And the mRNA and protein of EGFR, Duox1 and HO-1, the protein of p-EGFR increased significantly as compared to the control group. After the cells were pre-treated with hemin, the mRNA and protein of HO-1 increased significantly, while the mRNA and protein of Duox1, EGFR and MUC5AC, the protein of p-EGFR decreased significantly as compared to the CSE group. After the cells were pre-treated with ZnPPIX, the increase of HO-1 mRNA and protein were not significant as compared to the control group, while the mRNA and protein of Duox1, EGFR and MUC5AC, the protein of p-EGFR increased significantly. Conclusions HO-1 decreased the level of Duox1, blocked ligand-dependent EGF-R activation，decreased the expression levels of MUC5AC.

Clinicopathological features identify nonfamilial MSI-H colorectal cancer

Xiao-ming MENG; Jian-qiu SHENG; Zi-tao WU; Lei FU; He-juan AN; Ying HAN; Shi-rong LI

2010, 30(3):  252-257. 

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Objective According to the revised Bethesda guidelines, the relationship between clinicopathological features and microsatellite instability (MSI) testing in nonfamilial colorectal cancers patients was analyzed, Our aim was to identify clinicopathological features that identify high MSI (MSI-H).Methods We enrolled 150 patients, standard microsatellite loci (BAT25、BAT26、D2S123、D5S346、D17S250) were amplified by polymerase chain reaction（PCR）with fluorescent primers, and the PCR products were analyzed by GeneMapper software; age at diagnosis, gender and site, were obtained; various pathological features(tumor grade, mucinous differentiation, histologic heterogeneity, Crohn's-like response) were observed by light microscope; the express of tumor infiltrating lymphocytes(CD4+ and CD8+) was detected by immunohistochemistry. Using a stepwise logistic regression model, a formula was generated that could be used to calculate the probability of a colorectal carcinoma being MSI-H based on pathological features. Results Among 150 cancers, MSI-H was 13.33％;Independent identifier were poor differentiation, histologic heterogeneity , Crohn's-like reaction and tumor-infiltrating lymphocytes, logistic regression formula has a sensitivity of 70.0% and a specificity of 99.2% and a accurate ratio of 95.3% for MSI-H. Conclusions MSI-H phenotype cancer is a type of nonfamilial colorectal cancer with specific pathological features, Clinicopathological features can identify efficiently MSI-H colorectal cancers.

Effect of melatonin on the inhibition of connective tissue growth factor and remodeling in asthmatic mouse

Min WANG; Bei LI; Guang-huan ZHANG

2010, 30(3):  258-262. 

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Objective To explore the effcet of Melatonin（MT） on connective tissue growth factor(CTGF) and remodeling in asthmatic mouse.Methods The mice were randomly divided into 4 groups:control group,asthmatic group,MT treated group,Dexamethasone treated group,10 mice in each group. The mice were sensitized and repeatedly challenged with ovalbumin(OVA) for 2,4 weeks.MT treated group were intraperitoneal injected MT,and Dexamethasone treated group were intraperitoneal injected DXM.The eosinophil around the airway was observed after the histological section of lung staining with hematoxylin and eosin staining. Masson staining was used to stain collagen fibers and PAS staining was used to stain airway mucus.The following parameters were measured by Leica image analysis software:the perimeter of bronchial basement membrane（Pbm）,total bronchial wall area（WAt）,inner wall area（WAi）,smooth muscle area（WAm）,collagen area（Wcol）and mucus area. The protein expression of CTGF was assessed with immunohistochemistry. Results WAt/ Pbm、WAi/ Pbm、WAm/ Pbm 、Wcol/ Pbm 、the mucus area and the expression of CTGF were significantly higher in asthmatic group than those in control group. MT treated group and dexamethasone treated group could significantly alleviate the above-mentioned indicators increased. Conclusions MT could inhibit the expression of CTGF,and partly inhibit airway remodeling,which has a comparative potency of dexamethasone.

Exploring on the Prediction Model of Chronic Renal Failure based on Serum Proteomics

Lei HE; Ya-wei CHENG; Ping LIAO; Heng HU; Ya-ming JIN; Fu-feng LI; Wen-jing WANG; Peng QIAN; Yi-qin WANG

2010, 30(3):  263-267. 

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Objective To Screen serum protein markers related to CRF and establish the diagnosis model, exploring and discussing its significance in serodiagnosis by comparing differences of serum protein spectrum expression between patients with chronic renal failure（CRF） and control group.Methods Collecting 62 patients of CRF and control group with 28 normal ones.Serum samples were tested by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS)．The data were analyzed to screen serum proteomic biomarkers．By bioinformatics analysis ,decision classification tree models were to be established and tested.Results A total of 19 effective protein peaks were significantly different between CRF and normal control (P<0.001) at m/z range of 1500 to 30000，among which 18 showed low expression and 1 showed high expression in CRF. CRF and normal control was obviously different from the nature of the clustering; and samples of each group near each other, inter-group samples from each other. By the bioinformatics analysis , establishing a "CRF-normal controls " of the Diagnostic decision tree model, which was 87.8% in prediction accuracy rate with a sensitivity of 87.1% and a specificity of 89.3%.Conclusions Diagnostic decision tree model made more accurate judgments prediction and provided the experimental evidence for early clinical detection

Sequencing Analysis on rpoB Gene Mutation of Rifampin-resistance in Mycobacterium Tuberculosis

Xin GAN; Ling CHEN; Jian-hua WANG; Na-na LI; Yang CHEN; Jian-yong ZHANG; Hong ZHANG

2010, 30(3):  268-271. 

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Objective Genetic evaluation of relationship between rpoB gene mutation and rifampin-resistance in Mycobacterium Tuberculosis（MTB）. Methods This study was carried out on 81 clinical isolates with drug susceptibility testing and genotypic analysis by PCR amplification and sequencing of rpoB gene. Results Among these isolates, there were 27 rifampin-resistant and 54 susceptible strains. Of the 27 drug-resistant isolates, 20 (74.1%) carried mutations on the amplified fragment of the rpoB gene with 10 mutation types at seven codons including 531 and 526, and two new mutation patterns were recognized. On the other hand, one mutation (1.9%) appeared in 54 drug- susceptible strains. Conclusion The study showed geographical variation in the mutation types of rpoB gene in M. tuberculosis isolates from Guizhou of China, and this finding is valuable for the development of rapid molecular diagnostic methods suitable for specific regions.

Genetic Instability of P57kip2 Misexpression in Human Hepatocellular Carcinoma

Xiao-jia YI; Li-juan SHEN; Zong-ji ZHANG

2010, 30(3):  272-274. 

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Objective To study the expressions of P57kip2 mRNA and genetic instability of P57kip2 in human hepatocellular carcinoma (HCC).Methods In situ hybridization(ISH) was used to detect the expression of P57kip2 mRNA in HCC，MSI and LOH were detected by using PCR-Polyacrylamide gel electrophoresis-silver staining method.Results There was no expression of P57kip2 mRNA in normal liver tissue. The expression rate of P57kip2 mRNA both in pericancerous cirrhosis and hepatocellular carcinoma was 26.67﹪(8/30). LOH was not identified in 30 cases on three microsatellite locies；there were 2 microsatellite locies showing MSI，the total rate of MSI was 16.7﹪. There was correlation between the MSI of D11S1760 locies and the expression of P57kip2 mRNA(p<0.05).Conclusion The disorder expression of P57kip2 mRNA indicated that P57kip2 may be involved in hepatocarcinogenesis and prognosis. MSI may be one of the reasons that lead to the disorder expression of P57kip2 mRNA in hepatocarcinogenesis and prognosis.

Expression of tumor necrosis factor related apoptosis inducing ligand ( TRAIL) receptors on peripheral blood mononuclear cells in human chronic Hepatitis B patients and its clinical significance

Xiang-feng LENG; Zhen-yu CHEN; Qiu ZHANG; Wen-sheng SUN

2010, 30(3):  275-279. 

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Objective To explore the role of the expression of TRAIL receptors on peripheral blood mononuclear cell (PBMC) in the apoptosis of PBMC , and the relativity of the expression of TRAIL receptors with the severity of liver damage in chronic Hepatitis B patients. Methods The expressions of DR4、DR5、DcR1 and DcR2 in 55 cases of chronic hepatitis B patients and 30 cases of normal people were assayed by RT-RCR and Flowcytometery. The relativity of the expression of TRAIL receptors and the severity of liver damage were analized. Results The level of DcR1 in the PBMC of chronic B hepatitis patients was much lower than that of control group (p<0.05). There was close relation between the expression of DcR1 and liver damage (p<0.05). The level of DcR1 was negatively correlated with ALT, and positively correlated with serum albumin. Conclusion The expression level of DcR1 on PBMC in chronic hepatitis B patients was down-regulated, which might contribute to the increased apoptosis of PBMC in chronic B hepatitis patients. The expression of DcR1 can reflect the degree of liver injury in chronic hepatitis B patients to some degree.

Relationship Between Angiontensin II type2-receptor Gene polymorphism and Essential Hypertension in men

Chuan-fang LI; Dong-sheng GAO

2010, 30(3):  280-283. 

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Objective:To investigate the relationship between the AT2-receptor 1675A/G gene polymorphism and essential hypertension in men. Methods: The studied objects were born in Jining city 113 EH male patients are EH group , with 101 healthy people as control group.To gather clinical data and extract DNA from blood with reagent-case.The 1675A/G polymorphism of AGTR2 gene was detected by PCR , HRM and gene sequencing. Result: There were difference in genetypes(AA、GG) distribution and allele frenqency of AGTR2 1675A/G between male EH and control group(p＜0.05). Genotypes（AA、GG） distribution in EH group: 56（49.6％）,57（50.4％），is significantly higher than in control group, 32 (31.7%), 69 (68.3%), (p < 0.05). Allele frenqency in EH group: A:112（49.6%）and G:114（50.4%）, is significantly higher than in control group: 32（31.7％）and 69（68.3％）（p＜0.05）. Conclusion:The 1675A/G polymorphism of AGTR2 gene may be associated with male EH in Jining area ，Shandong province.

Effects of RNA interference targeting HIF-1αon location and metastasis in HeLa cells

Wei-guang WU; Ya-qiong CHEN; Xiu-qin CAO

2010, 30(3):  284-288. 

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Objective To construct eukaryotic vector expressing short hairpin RNA(shRNA) of HIF-1α,and to observe its effects on location and metastasis of HeLa cells under hypoxic condition. Methods shRNA templates was designed based on HIF-1αgene sequence and was cloned into pSilencer2.1-U6-neo vector. The resultant plasmid was transfected into HeLa cells with Lipofectamine 2000. The cells were incubated under hypoxic condition. The HIF-1αprotein and mRNA were detected by Western blot and real time PCR. The colony formation assay and Transwell cabin assay were performed to measure the colony formation and metastasis. Results The plasmid pSilencer2.1-U6-neo-HIF-1α was successfully constructed and transfected into HeLa cells. The expression of HIF-1αin HeLa cells decreased, and the number of colony formation in soft agar and cells penetrating matrigel also decreased under hypoxic condition. Conclusions The shRNA expressing plasmid targeting HIF-1αcould suppress the location and metastasis of cervical carcinoma cells under hypoxic condition.

Erythropoietin reduces myocardial ischemia-reperfusion injury in rats

Jin-cun GUO; Wei-bin HUANG; Yi-qing WANG; Liang-di XIE

2010, 30(3):  289-292. 

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Objective To investigate the influence of erythropoietin in the metabolism of extracellular matrix after myocardial ischemia-reperfusion injury. Method The langendroff reperfusion system was applied to investigate the protect function of EPO on the ischemia-reperfusion condition in rats. The effects of EPO on extracellular matrix were observed by western blot and signal pathway blocker. The LVDP and infarction area were observed at the same time. Results: EPO significantly improved LVDP（19.8+0.2mmHg vs 35.9+0.2mmHg，IR group vs EPO+IR group，p<0.05）and decreased infarction area （35.26%+7.13% vs 62.70%+7.23% IR group vs EPO+IR group，p<0.05）. The MMPs expressions were significantly attenuated and Collagenase I/III were significantly enhanced by EPO(MMP2 53.2+2.6vs21.2+2.5; MMP9 57.6+3.1vs 19.2+2.6; IR组vs IR+EPO组, p<0.05 ；CollagenI 43.2+2.2vs11.4+2.3; CollagenIII 55.3+3.2vs 18.1+2.3; IR+EPO组vs IR组, p<0.05 ). This function can be inhibited by Mek-Erk inhibitor. Conclusion: EPO play a role in the metabolism of extracellular matrix by Mek-Erk signal pathway, which can protect the heart tissue from ischemia-reperfusion injury.

Utility of a multiprobe fluorescence in situ hybridization assay in the detection of bladder cancer

Xue-fei DING; Han-zhong LI; Wei-gang YAN

2010, 30(3):  293-296. 

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Objectives To evaluate the clinical utility of a Multiprobe FISH (multicolor fluorescence in situ hybridization, M-FISH) assay in voided urine specimens for the detection of bladder cancer, comparing the results with those afforded by urinary cytology. Methods Voided urine specimens from 100 patients who had cancer tumor or after surgery and 10 patients who began prostate hyperplasia were obtained for urinary cytology and FISH. FISH was performed using a mixture of fluorescent labeler DNA probes for the centromeric regions of chromosomes 3, 7, and 17 and 9p21 region. Cystoscopy with biopsy or tumor resection was performed in all patients, comparing the pathological results with the cytological and FISH findings. Results The sensitivities for M-FISH were 75.6% for low grade tumors, 100% for high grade tumors, with overall sensitivities of 85.5%. Urinary cytology affords an overall sensitivity of 62.9%, the figure being 33.3% for low grade tumors, 96.0%for high grade tumors. Significant difference in overall sensitivities was observed between M-FISH and Urinary cytology (P<0.05). The specificities of M-FISH and urinary cytology were 84.6% and 87.8%, respectively. No significant difference in specificities was observed between M-FISH and Urinary cytology .Conclusion M-FISH improves the sensitivity rates obtained with urinary cytology for bladder cancer detection, and affords similar specificity, and can detect all high grade infiltrating tumors patients.

Analysis of drug-resistant genes of 60 Acinetobacter baumannii isolates

Yu-ping WANG; Yan-rong SONG; Ji-hong LI; Ai-xiang LIU

2010, 30(3):  297-302. 

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Objective To investigate β-lactamase gene and class 1 integron gene of 60 clinical Acinetobacter baumannii isolates. Methods The minimal inhibitory concentrations (MICs）for 16 antibiotics widely used in clinic were determined using the standard broth microdilution method. The β-lactamase gene, class 1 integron gene and adeB gene were determined by PCR and were then sequenced. Results Fifty-three strains of the 60 A. baumanii isolates were multi-drug resistant. OXA-23 gene was detected positive in six A. baumanii isolates, which were all resistant to more than five antimicrobial agents including carbapenem and showed high resistance to many antibiotics. The 38 strains that carried PER-1 gene showed higher resistance to cephalosporins than those without this gene (P<0.01). Class 1 integron gene was positive in 45 strains, which exhibited significantly higher multiple resistance than those without this gene (P<0.01). The 25 strains carrying both class 1 integron and PER-1 genes had a markedly higher multiple resistance (P<0.01), but not in resistant level, compared to the 7 strains without these two genes. Conclusion Class 1 integron and β-lactamase gene may be the causes of multidrug-resistance of A. baumanii. The strains carrying OXA-23 gene always showed multiple and high resistance to several antibiotics, so effective measures must be taken to control the epidemic of these strains.

IL-6 can upregulate porcine ovarian granulosa cells androgen receptor expression

Ling HONG; Xiao-ming TENG; Kun-ming LI; Wen-qiang HUANG; Yan ZHANG; Bo ZHANG; Xiao-wen TONG

2010, 30(3):  303-305. 

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Objective To study the effect of IL-6 on the expression of androgen receptor (AR) in porcine ovarian granulosa cells(GC) and to explore the role of interleukin 6 (IL-6) in the polycystic ovary syndrome pathogenesis. Methods Different concentrations of IL-6（10、100 and 1000ng/L） were treated with GC. Expression of androgen receptor (AR) mRNA and protein in GC were detected by Real-time Quantitative RT-PCR and Western blot. Results The mRNA and protein expressions of AR in GC were upregulated in groups with 100 and 1000ng/L IL-6 at 72h. Conclusion IL-6 can improve androgen activity. This process may explain the function of chronic subclinical inflammation in PCOS pathogenesis.

临床园地

A clinical evaluation of anesthesia for patients with myasthenia gravis using target controlled infusion without muscle relaxant

Jie YI; Yu-guang HUANG; Ai-lun LUO

2010, 30(3):  306-308. 

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Objective To investigate the safety and feasibility of anesthesia for patients with myasthenia gravis using target controlled infusion without muscle relaxant. Methods 31 patients with myasthenia gravis were involved into study. A target controlled infusion was started with targeting effect-site concentration of propofol 3μg/ml and remifentanil 4ng/ml. Intubation was performed when patients were unconsciousness and target concentrations of both drugs were reached. No muscle relaxant was used during anesthesia. Blood pressure,heart rate, performance of intubation and respiratory recovery including extubation and wake time were observed. Result All patients were intubated successfully in one attempt. 38.7% patients were present mild cough when the endotracheal tube past through the vocal cord during intubation. Blood pressure and heart rate at post-intubation increased significantly compared with pre-intubation (P<0.01). After cease of drugs, time of spontaneous breathing recovery was 6.5±2.9min. Extubation and wakeup time were 9.8±3.6 and 7.4±3.1min respectively. No adverse event was noted. Conclusion Target controlled infusion without muscle relaxant was safe and effective anesthesia for myasthenia gravis patients undergoing thymectomy.

研究短文

Effects of Endothelin-1 on the expression of laminin in human glomerular mesangial region

Zhi-fang HUANG; Li-de LUN; Chi-bing ZHANG; Kang JI

2010, 30(3):  309-310. 

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Expressions of Livin and Survivin and their clinical significance of pathology in Breast Cancinoma

Jian-hua CHEN; Shi-hai ZHU; Xiu-di XIAO; Qin-he FAN

2010, 30(3):  311-312. 

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Carvedilol delay ventricular remodeling after myocardial infarction in rabbits

Zhi-bin HONG; Su-sheng WEN; Yuan-zheng ZHANG; Hong SHANG

2010, 30(3):  313-314. 

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Inhibitory effect of urotensinII on L-type calcium current of ventricular myocytes in rat

Yong LIU

2010, 30(3):  315-316. 

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短篇综述

Rituximab treatment of systemic lupus erythematosus associated pulmonary arterial hypertension: a new insight

Wei WANG; Qian WANG; Jiu-liang ZHAO; Meng-tao LI; Xiao-feng ZENG

2010, 30(3):  317-319. 

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Pulmonary arterial hypertension（PAH） is one of the most severe complications of systemic lupus erythematosus（SLE）, recent studies have emphasized the role which T and B lymphocytes play during the pathogenesis of PAH. Rituximab（RTX）, not only targeting at B lymphocytes, but also been proven to affect T cells, thus could be a potential treatment for SLE-PAH.

Preparation and Application of Multicellular Spheroid in Cancer Research

Qiang XU; Quan LIAO; Yu-pei ZHAO

2010, 30(3):  320-324. 

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Multicellular spheroid (MCS) can mimic many aspects of the in vivo physiological and pathological conditions, better reflect the in-vivo behavior of cells in tumors. So it's increasingly recognized as a valuable tool for evaluating the efficacy of therapeutic intervention including chemotherapy, radiotherapy, immunotherapy and combined therapy. Various spheroid co-culture approaches have been presented to study heterologous cells interaction in solid tumors. The present review briefly introduces the methodology and applications of MCS, and focuses on the recent advances during these years.

Mitogen-activated protein kinases and morphine tolerance

Yan-bo ZHANG; Bao-liang SUN; Hui YUAN; Ming-feng YANG; Jing-zhong NIU; Guo-wei LV

2010, 30(3):  325-328. 

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The MAPKs activation pathway consists of three protein kinases in activation sequence: MAPKs kinase kinases (MKKKs) →MAPKs kinases (MKKs) →MAPKs and four pathways:extracellular signal-regulated kinase (MAPKERK), c-Jun N-terminal kinase (MAPKJNK), MAPKP38 and MKK5/MAPKERK5 activation pathways.It has been proved that MAPKs(ERK,JNK and P38) are acvtivated in the progress of morphine tolerance. Inhibitors of each and every element of MAPKs activation pathway are promising clinical drugs for morphine tolerance.

Research progress on the effect of hydrogen in the treatment of ischemia-reperfusion injury

Chen XU; Run-ping LI

2010, 30(3):  329-332. 

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Ischemia-reperfusion injury is common in clinical treatment. Free radical induced oxidative stress plays an important role in this process. Recently, it is reported that hydrogen can selectively scavenging hydroxyl radical in rodents，thus alleviate ischemia-reperfusion injury. As hydrogen is easy to penetrate the barriers in the body and has little adverse effects to body, it is hopefully to become a promising agent against ischemia-reperfusion injury in the future.

The Biological Significance of Endothelial Progenitor Cells in Angiogenesis and Vascular Tissue Engineering

Gui-lin LI; Bao-zhong YANG

2010, 30(3):  333-336. 

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Endothelial progenitor cells (EPCs) can differentiate into mature endothelial cells and participate in postnatal vascular regeneration and impared endothelium repair. Researches in recent years on use of EPCs as seed cells in promoting angiogenesis, maintaining the integrity of endothelial function and constructing tissue engineered blood vessels are reviewd.