Abstract: Objective To study hIGF-1gene expression and the influence on aggrecan expression of degenerative intervertebral disk. Methods twenty four male New-Zealand rabbits IDD models were done according to reference[1] and randomly divided into Ad/CMV-hIGF-1、hIGF-1 growth factor and PBS group. Twenty five microlitre the second generation Ad/CMV-hIGF-I（T=80×109 PFU/L）, hIGF-I growth factor(100 μg/ L) and PBS were respectively injected into L4-5, L5-6 intervertebral disk under fluoroscopic guidance. One，two，four and eight weeks post-operation, rabbits were sacrificed, intervertebral disk samples were harvested. Total proteins of equal mass intervertebral disks were extracted, isolated in SDS--polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) Millipore. The hIGF-I growth factor expression were indentified with Western blot. Aggrecan gene fragments were amplified with RT-PCR，and relative expression was done with GAPDH as intern control. Results The hIGF-I interest protein existed at 7.6 kilo-Dalton. At one week after injection, its expression quantities were almost equal between Ad/CMV-hIGF-I and hIGF-I growth factor group. At two week after injection, it obviously declined in hIGF-I growth factor group. At four week after injection, it still expressed in Ad/CMV-hIGF-I group. At eight week after injection, it did not express in three groups. Aggrecan gene relative expression increased significantly from one to four weeks after injection, declined slightly at the end of eight weeks in Ad/CMV-hIGF-I group. However, it appeared to decrease continuously in the other two groups with time. At the corresponding phases, that in PBS group were the lowest. Conclusions Ad/CMV-hIGF-I successfully infected degenerative intervertebral disk. The hIGF-I gene expression lasted four weeks and could stimulate aggrecan synthesis in Ad/CMV-hIGF-I group.