Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (6): 618-621.

• 研究论文 • Previous Articles     Next Articles

Modulation of Th1 and Th2 responses to immunization with different β-amyloid and adjuvant

Tian-tian LEI, Xue-mei ZHAO, Ping LIANG   

  1. Department of Pathology, School of Basic Medicine , IBMS, CAMS&PUMC Department of Pathology, School of Basic Medicine , IBMS, CAMS&PUMC
  • Received:2009-03-10 Revised:2009-04-08 Online:2009-06-25 Published:2009-06-25
  • Contact: Ping LIANG

Abstract: Objective To observe stimulation and regulation of T lymphocyte subtypes when using wild-type and mutant Aβ40 and Aβ42 with different adjuvants and to investigate possible ways to lower the toxicity of Aβ immune response. Methods Forty BALB/c mice were randomized into five groups: Al adjuvant group,Aβ42+CFA group,Aβ42+Al group,Aβ40+Al group,Aβ40(E22A)+Al group. After the initial immunization and booster immunization 3 times, antibody titer were detected and the mice were killed. Their splenocytes were stimulated by respective antigen. After 48h quantity of IFN-γ, IL-2, TNF-α and IL-4 were tested. After 72h of culture, the proliferative response of splenocytes were detected by CCK-8 assay. Results Experimental groups generated specific anti-Aβ antibodies and had proliferative response of spleen lymphocytes. Cell culture supernatant of cytokine detection results showed that Aβ42+CFA group had the highest secretion of Th1-type cytokines IFN-γ,IL-2,TNF-α when Aβ40(E22A)+Al group had the lowest.(P <0.01).When Aβ40 and the mutant Aβ40+Al groups compared with Aβ42+Al group, secretion of IFN-γ,IL-2,TNF-α have also decreased significantly(P<0.05).The secretion of IL-4 was no significant difference. Conclusion Aβ40(E22A)+Al group stimulate the lowest toxicity of T cells, and may have better security in the immune response.

Key words: Alzheimer’s disease, Aβ peptide, T cell subtypes, cytokine