Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (8): 1230-1236.doi: 10.16352/j.issn.1001-6325.2022.08.1230

• Original Articles • Previous Articles     Next Articles

miR-499 inhibits migration and promotes apoptosis of human osteosarcoma cell line Saos2 by targeting at TGF-α

WANG Lei1, QIU Ming-xian1, ZHANG Hui-rong1, ZHANG Jin-ping1, ZHAO Jing1, KANG Xiao1*, ZHANG Qing-quan2   

  1. 1. the Fourth People's Hospital of Hengshui, Hengshui 053000;
    2. Hengshui People's Hospital of Hebei Province,Hengshui 053000, China
  • Received:2021-06-01 Revised:2021-11-22 Online:2022-08-05 Published:2022-08-01
  • Contact: *

Abstract: Objective To explore the role of miR-499 targeting TGF-α mRNA protein in regulating the migration and apoptosis of human osteosarcoma cell line Saos2 and its pathogenesis. Methods The expression differences of miR-499 and TGF-α in osteosarcoma tissues were detected by RT-qPCR. Double luciferase reporter gene assay was used to detect the targeting relationship between miR-499 and TGF-α. Transwell cell assay and TUNEL apoptosis assay were used to detect the migration and apoptosis of Saos2 cells. A nude mouse xenograft model of osteosarcoma was constructed to detect the regulatory mechanism of miR-499/TGF-α axis in vivo. Results In osteosarcoma, miR-499 expression was decreased (P<0.01) but TGF-α mRNA expression was increased(P<0.01). miR-499 negatively regulated TGF-α mRNA protein expression (P<0.01). Transfection of miR-499 simulacrum inhibited the migration and promoted apoptosis of Saos2 cells (P<0.01), while TGF-α promoted the migration and inhibited apoptosis of Saos2 cells (P<0.01). miR-499 inhibited the carcinogenic effect of TGF-α. Conclusions miR-499 inhibits the biological function of osteosarcoma cell line Saos2 by inhibiting at TGF-α mRNA protein level which suggests that miR-499 may inhibit the development of osteosarcoma.

Key words: osteosarcoma, miR-499, TGF-α, Saos2

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