Basic & Clinical Medicine ›› 2017, Vol. 37 ›› Issue (2): 211-216.
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Abstract: Objective To investigate the relationship of ELK-3 and epithelial-mesenchymal transition (EMT),exploring its possible mechanism. Methods The human hepatocellular carcinoma cells (HCC) were divided into small interference RNA transfection group and Ras-ELK-3 pathway inhibitor group. The protein levels of ELK-3 target gene EGR-1 E-cadherin,vimentin and p38 in HCC) were determined by Western blot analysis. Results The protein levels of ELK-3 and its target gene EGR-1 in treated human hepatocellular carcinoma cells were decreased significantly as compared with the negative control group (P < 0.01). The protein level of E-cadherin was significantly increased (P < 0.01),while vimentin and p38 were decreased in HCC cells with ELK-3 interference (P < 0.01). Conclusions ELK-3 interference can inhibit the epithelial- mesenchymal transition of HCC cells by down-regulating p38.
Key words: ELK-3, hepatocellular carcinoma, epithelial-mesenchymal transition, p38
CLC Number:
R735.7
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2017/V37/I2/211