Abstract: Objective The study was designed to explore the possible role of magnoIoI against myocardial ischemic damage and several related signaling pathways as potential mechanisms. Methods The protective properties of magnoIoI were studied in a rat model of acute myocardial infarction due to permanent ligation of the left anterior descending coronary artery. MDA content and SOD activity was measured by ELISA method,Apoptosis of cardiomyocytes was also observed by TUNEL staining.the expression of Bcl-2, Bax and caspase-3 were examined by Western blot.Intracellular calcium concentration [Ca2+]i was tested Laser scanning confocal microscopy. Results The results showed that administration of magnoIoI relieved myocardial injuries during ischemia, and this were achieved by protecting cardiomyocytes from apoptotic death. The beneficial effects of magnoIoI were likely mediated by activation of the survival signaling molecule (Bcl-2), and a reduction of apoptotic mediator (Bax, caspase-3) and intracellular Ca2+ overload. Conclusion the findings showed that administration of magnoIoI relieved myocardial injuries duringischemia, and this was achieved by protecting cardiomyocytes from apoptotic death.

Key words: KEY WORDS magnoIoI, myocardial ischemic, Bcl-2, Bax, Caspase-3