Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (3): 345-349.
Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Contact:
Abstract: Objective To investigate the role and mechanism of ERK1/2 MPAK pathway in regulating proliferation and migration of Eca109 cell line in vitro. Methods We treated Eca109 cells with MAPK(ERK1/2)inhibitor(U0126); cell counting method detected the cell proliferation; Cell morphology was observed under inverted microscope;qRT-PCR detected ERK1/2 mRNA level; Western blot determined the expression of ERK1/2 protein level; MTT and Wound scratch assay detected cell proliferation and migration; qRT-PCR examined miR-21 expression. Results Cell growth was inhibited by U0126 at 20μmol/L doses(p<0.05), destroyed the normal cell morphology; inactivited ERK1/2 MPAK pathway at protein level(p<0.05); inhibition of ERK1/2 MPAK pathway repressed cell proliferation and migration of Eca109 cells and downregulated the expression of miR-21(p<0.05). Conclusion Inhibition of ERK1/2 MPAK pathway weakened cell proliferation and migration of Eca109 cells, one of the most possible mechanism is associated it’s inhibition role on miR-21 expression.
Key words: Key words: ERK1/2, Eca109 cell, proliferation, migration, MicroRNA-21
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2014/V34/I3/345