Abstract: Objective To observe the effect of adenomatous polyposis coli (APC) on cell mitochondria in the process of fat metabolism in human hepatoma cell lines. Methods Human hepatoma cells (BEL-7402) cells were transfected with APC gene knockdown plasmid, and oleic acid (OA) was used to stimulate knockdown cells and control cells to produce lipid accumulation. Oil red O staining was used to detect the lipid accumulation in each group. ATP content detection kit was used to detect the ATP content of each group. The content of oxygen free radicals (ROS) in each group was detected by fluorescent probe labeling. Results After transfection of the plasmid, the mRNA expression of APC gene decreased (P<0.05) and protein expression decreased (P<0.05). Reduced cell lipid accumulation after stimulation with oleic acid in APC knockdown group compared with control cells (P<0.05), ATP content increased (P<0.05), and the number of intracellular oxygen free radicals decreased (P<0.01). Conclusions APC affects the physiological function of mitochondria during lipid metabolism in human hepatoma cell lines.

Key words: Key words: fatty liver cells, APC, mitochondria