Abstract: The pathogenesis mechanism of osteosarcoma closely related to the expression inactivation or even loss of multiple tumor suppressor genes, including PTEN, WWOX, WIF-1, SPARCL1, p53 and so on, which inhibited the growth of osteosarcoma. Methylation was one of the important epigenetic features, and the relevant studies about the methylation of these tumor suppressor genes provided valuable or fundamental support to clinical diagnosis, treatment and pathogenesis of osteosarcoma, which also offered an ingenious idea to develop new anti-osteosarcoma drugs.

Key words: tumor suppressor gene, methylation, osteosarcoma, treatment