Abstract: Objective To investigate the effect of resveratrol on high glucose-induced oxidative stress and cardiomyocyte hypertrophy, together with its possible mechanism. Methods H9C2 cardiomyocytes were divided into normal glucose group, high glucose group, resveratrol group and resveratrol + DAPT group, respectively. The cell surface area was measured by rhodamine-labeled phalloidin. Reactive oxygen species generation was measured using DCFH-DA. The contents of SOD and MDA were evaluated by colorimetry. The mRNA and protein levels of Notch1, hes1, ANP and BNP were evaluated by RT-qPCR and western blot. Results Compared to normal glucose group, high glucose exposure significantly increased cell surface area（p < 0.01）and enhanced ROS and MDA generation（p < 0.01）with concomitant decreased SOD activity（p < 0.01）, as well as decreased the mRNA and protein expressions of Notch1 and hes1（p < 0.05）, and increased the expression levels of ANP and BNP（p < 0.05）. Resveratrol treatment suppressed increased cell surface area（p < 0.01）and decreased ROS and MDA generation（p < 0.01）with concomitant enhanced SOD activity（p < 0.01）, as well as increased the expression levels of Notch1 and hes1（p < 0.05）, and decreased the expression levels of ANP and BNP（p < 0.05）. Conclusions Resveratrol may inhibit high glucose induced H9C2 cardiomyocytes hypertrophy by improving oxidative stress and activating Notch1/hes1 signaling pathway．

Key words: Key words: resveratrol, diabetic cardiomyopathy, Notch1, hypertrophy